Figure 1.
Defense mechanism of Zostera marina.
Table 1.
Sampling sites of Zostera marina.
Figure 2.
Experimental design and setup of experiment I and II.
Table 2.
Experiment 1: Statistical analysis of differences in Labyrinthula zosterae abundance, lesion size, growth rate and leaf production after inoculation of Zostera marina with L. zosterae compared with uninoculated plants.
Figure 3.
Abundance of Labyrinthula zosterae cells per mg Zostera marina leaf sample (dry weight) depending on inoculation time during experimental L. zosterae infection.
Results are partly from experiment I and II, means with standard error bars.
Table 3.
Zostera marina genes for gene expression analysis and their predicted function.
Table 4.
Experiment II: Statistical analysis of gene expression in Zostera marina after inoculation with Labyrinthula zosterae depending on inoculation time.
Figure 4.
Growth (a) and leaf production (b) of Zostera marina leaves 2–4 weeks after experimental infection with Labyrinthula zosterae.
2nd leaf = inoculated 2nd oldest leaf of each Zostera marina shoot (growth measured 1st to 2nd week post inoculation), 1st leaf = youngest leaf at inoculation, not inoculated (growth measured 1st to 4th) week post inoculation), leaf 0 = leaf not yet present at inoculation, therefore not inoculated (growth measured 3rd to 4th week post inoculation). * indicates significant differences at p<0.05, *** indicates significant differences at p<0.01, ns = not significant, means with standard error bars.
Figure 5.
Abundance of Labyrinthula zosterae cells per mg Zostera marina leaf sample (dry weight) after experimental inoculation depending on the parental site of Z. marina (a) and the isolation site of L. zosterae (b).
*** indicates significant differences at p<0.01, ns = not significant, means with standard error bars.
Figure 6.
Spread of lesions on Zostera marina 2nd oldest leaves of different origin after experimental inoculation with Labyrinthula zosterae, *** indicates significant differences at p<0.01, means with standard error bars.
Figure 7.
Gene expression of Zostera marina defense genes after experimental infection with Labyrinthula zosterae.
I = inoculation treatment with L. zosterae, NI = no inoculation. Results have been normalized to eIF4A housekeeping gene. −ΔCt: log 2 scale. * indicates significant differences at p<0.5, ns = not significant. RPPA: NB-ARC domain-containing disease resistance receptor gene. EDS-5: Enhanced Disease Susceptibility 5. Met: Metacaspase APX: L-ascorbate peroxidase. GST: Glutathione S-transferase. CAT: catalase II. SOD: superoxide dismutase. HSP70: heat shock protein 70. Prot-206: Disease resistance-responsive protein 206. Chit: Chitinase. CYP73A: Trans-cinnamate 4-monooxygenase, means with standard error bars.