Table 1.
Descriptions of candidate reference genes in O. javanica.
Figure 1.
Cq values of candidate reference genes in all samples of O. javanica.
The line across the box depicts median. The inside box depicts mean. The outside box is determined by the 25th and 75th percentiles. The whiskers are determined by the 5th and 95th percentiles. The asterisk represents outlier.
Figure 2.
Determination of the optimal number of reference genes required for effective normalization.
Pairwise variation (Vn/n +1) analysis between the normalization factors (NFn and NFn +1) was performed by the geNorm program to determine the optimal number of reference genes, and carried out for qPCR data normalization in various sample pools. GA: gibberellin; SA: salicylic acid; MeJA: methyl jasmonate; ABA: abscisic acid; Abiotic stress: heat, cold, salt, and drought; Hormone stimuli: SA, GA, ABA, and MeJA; Total: all sample.
Table 2.
Gene expression stability under individual stress ranked by geNorm, NormFinder and BestKeeper.
Table 3.
Gene expression stability under multiple stress ranked by geNorm, NormFinder and BestKeeper.
Figure 3.
Relative quantification of M6PR gene expression using validated reference genes for normalization under salt treatment in O. javanica.