Table 1.
Summary of illumina sequencing from salmon lice Caligus rogercresseyi transcriptome.
Figure 1.
Gene Ontology terms distribution of BLAST hits from the C. rogercresseyi transcriptome.
Selected GO categories are shown with the top-level division of biological process, molecular function, and molecular function.
Figure 2.
Gene expression profiles during larvae stages and adults of C. rogercresseyi generated by high-throughput transcriptome sequencing.
(A) Overview of log2 expression ratios of all transcripts differentially expressed from nauplius I, nauplius II, copepodid, chalimus, adult females and adult males. (B and C) Two patterns of expression were detected by K-means algorithm using transformed expression values.
Figure 3.
Heat map generated from C. rogercresseyi transcriptome data reflecting gene expression values in six developmental stages.
Dendrograms of the transcription patterns were estimated for 83,444 contigs generated by de novo assembling. The bar color reflects the gene expression levels.
Figure 4.
Clusters of gene expression levels between Nauplius I–II and copepodid stages of C. rogercresseyi.
Dendrograms of the transcription patterns were estimated for 83,444 contigs generated by de novo assembling. The bar color reflects the gene expression level from black (low), red (medium) to yellow (high). Contig annotations of these 4 clusters are listed in Table S2.
Figure 5.
Clusters of gene expression levels between copepodid and chalimus stages of C. rogercresseyi.
Dendrograms of the transcription patterns were estimated for 83,444 contigs generated by de novo assembling. The bar color reflects the gene expression level from black (low), red (medium) to yellow (high). Contig annotations of these 5 clusters are listed in Table S3.
Figure 6.
Clusters of gene expression levels between adult females and males of C. rogercresseyi.
Dendrograms of the transcription patterns were estimated for 83,444 contigs generated by de novo assembling. The bar color reflects the gene expression level from black (low), red (medium) to yellow (high). Contig annotations of these 6 clusters are listed in Table S4.
Table 2.
Summary of clusters between Nauplius I–II/Copepodid, Copepodid/Chalimus, and Female/Male groups from C. rogercresseyi transcriptome.
Table 3.
Genes showing differential transcription expression between Copepodid/Nauplius I–II groups.
Table 4.
Genes showing differential transcription expression between Chalimus/Copepodid groups.
Table 5.
Genes showing differential transcription expression between Male/Female groups.
Figure 7.
Radar plot of contigs with significant expression values (P≤10–16; |fold-change|>5) in terms of percentages for nauplius I–II/copepodid, copepodid/chalimus and female/male from C. rogercresseyi.
Annotated contigs were associated to (i) aminoacid transfer, repair and breakdown, (ii) cellular process, (iii) cytoskeletal and contractile elements, (iv) molting cycle, (v) metabolism, homeostasis, mitochondrial genes, (vi) neuronal involvement and nervous system, (vii) reproduction, (viii) cell development and morphogenesis, (ix) Transcription and translation and (x) others.