Table 1.
Primers used for RT-qPCR analysis.
Figure 1.
Comparison of the morphology of DU145 cells treated with DTX, OCT alone and the two-drug combination for 48 h.
A. Untreated cells; B. 1 nM DTX; C. 100 nM OCT; D. 10 nM DTX; E. Two-drug combination of 10 nM DTX and 100 nM OCT; F. 100 nM DTX.
Figure 2.
Inhibition of cell growth by different concentrations of DTX (A), OCT (B) after 72 hours incubation and in a time- and dose-dependent manner both of them respectively (C and D) on DU145 proliferation.
Cell viability was determined by MTT assay and expressed as a percentage of the control value (mean ± SEM of three experiments done in triplicate); error bars = SEM (n = 6).
Figure 3.
Effect of DTX alone, synergistic effect of DTX in combination with 100(Fig. 3A) and various concentrations of two agents (Fig. 3B) on DU145 cells proliferation following 72 h of treatment.
Results are the percentage of control value obtained with untreated cells (mean ± SEM of three experiments done in triplicate). (**) Proliferation was significantly decreased (p<0.001); error bars = SEM.
Figure 4.
Effects of DTX, OCT alone and the combination of DTX/OCT on the mRNA expression of VEGFA, caspase 9, caspase 3 and ABCB1 in DU145 cells.
M: Marker, The expression levels of objective genes were examined by RT-PCR (A, B, C and D) and quantitative real-time -PCR (B, D, F and H) respectively, using cells treated with the test drugs for 72 hours. The expression level of each mRNA was normalized to the level of β-actin mRNA. Values represent the means±SD of triplicate analyses (*p<0.05, **p<0.01).
Figure 5.
Effect of DTX, OCT alone and two-drug combination treatment on DU145 cells apoptosis.
Data are mean ± SEM of two independent experiments performed in triplicate. ***p<0.000 vs. control.
Figure 6.
Effects of DTX, OCT alone and two-drug combination on DU145 cells migration.
(A) Wound healing assay to determine the effect of indicated drugs on DU145 cells migration. (B) The summarized migration ratio (%) following 72 h treatment measured by wound healing assay. Each column represents the mean ± SEM. **p<0.01, vs. control.