Table 1.
Anthropometric Characteristics of Participants in the Single Endurance Exercise Bout.
Table 2.
Clinical and Muscle Fiber Characteristics Before and After the Single Endurance Exercise Bout.
Figure 1.
Epinephrine and lipolytic cocktail (PFI) in human primary myotubes increases perilipin 3 and coatomer proteins.
A) Primary human skeletal muscle myotubes (n = 5) were stimulated with 100 uM of epinephrine. PLIN3 expression as assessed by densitometry increased continuously with epinephrine. Additionally, GTPases ARF1 and GBF1 increased 30 minutes following epinephrine stimulation and ARFRP1 increased 1 hour following epinephrine stimulation. B) PLIN3, ATGL ARFRP1, GBF1 and ARF1 protein content increased after in vitro lipolytic stimulus (PFI) in primary human skeletal muscle myotube (n = 5).
Figure 2.
Coatomer GTPase gene expression changes with lipolytic cocktail (PFI) in human primary myotubes.
mRNA level of Sec23a, ARF1 and GBF1 in cultured human myotubes before and after PFI treatment (0, 30 min and 1 h) (n = 5). *p<0.05
Figure 3.
Changes in protein and gene expression relating to lipolysis with a single bout of endurance exercise in human skeletal muscle.
A) Representative blots of PLIN3, ATGL, ARF1, ARFRP1 and loading control GAPDH. B) Quantitative bar graph of skeletal muscle PLIN3 protein after an acute exercise bout (n = 19). C) Quantitative bar graph of skeletal muscle ATGL protein after an acute exercise bout (n = 19). D) mRNA levels of lipid droplet coatomer genes (n = 19), and (E) mRNA levels of oxidative genes, in skeletal muscle of healthy subjects in response to an acute exercise bout (n = 14–19). *p<0.05.
Figure 4.
Associations between change in perilipin 3 (PLIN3) protein in skeletal muscle tissue and fat oxidation.
A) Correlation between the change in PLIN3 protein expression and the change in ex vivo palmitate oxidation measured from skeletal muscle tissue homogenates (n = 18). B) Correlation between the percent change in PLIN3 protein expression and whole body cumulative fat oxidation measured by indirect calorimetry adjusted for fat free mass (n = 18). C) Correlation between the change in total glycogen content and the change in PLIN3 protein during exercise (n = 18).