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Figure 1.

Experimental design.

A: Flow diagram. B: Timeline of events during one fMRI run (total duration 35 min). Every block represents one 4.5-min time bin. At all illustrated time points (t = min) blood was drawn and fullness, desire to eat and anxiety were rated.*During this time bin chocolate milk was infused or ingested, or water was infused.

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Figure 2.

Effect of gastric infusion of water and chocolate milk on brain activity compared to baseline.

Left panel: T-map of the increased response to chocolate milk and water infusion versus baseline overlaid onto the mean anatomical scan, thresholded at P<0.05, FWE-corrected for multiple comparison. Right panel: Mean parameter estimates (a.u. ± SEM) within significant clusters for water and chocolate milk infusion.

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Table 1.

Effect of gastric infusion on brain activation in healthy normal-weight young men.

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Figure 3.

Changes in brain activity after treatment for the three conditions. Left panel: T-map of the increased response to oral chocolate milk stimulation after administration versus baseline overlaid onto the mean anatomical scan, thresholded at P<0.05 (FWE-corrected for multiple comparison).

Right panel: Mean parameter estimates (a.u. ± SEM) over time from selected significant clusters. Area under the curve was greater for the oral condition in all brain areas, and for the control condition in the putamen (all P<0.05).

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Table 2.

Effect of treatment with chocolate milk on brain activation in healthy normal- weight young men.

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Figure 4.

Correlation between fullness and insulin changes (from baseline) and changes in brain activity in corresponding time bins during the gastric condition (n = 14, 5 time bins per subject, T-maps are thresholded at P<0.001, uncorrected for multiple comparisons).

Left pane: T-map of selected significant correlations overlaid onto the mean anatomical scan. A: Correlation T-map and scatter plot showing the parameter estimates of the ACC peak voxel at MNI (−6, −16, 30) against fullness changes. B: Correlation T-map and scatter plot showing the parameter estimates of the putamen peak voxel (34, 0, 6) against insulin changes. C: Correlation T-map and scatter plot of the parameter estimates of the insula peak voxel at MNI (−38, 0, 2) plotted against insulin changes.

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