Figure 1.
Experimental protocol, representative pictures of colon tumors and photomicrographs showing the pathological characteristic.
(A) Experimental procedures. (B) Normal colon. (C) Adenoma (white arrow). (D) Adencarcinoma (yellow arrow). The bloodstain around the tumor was caused by tumor ulcer instead of our dissection. Representative photomicrographs showing the normal mucosa (E), adenoma (F) and adenocarcinoma (G) were magnified in 40×(left), and each of the right micrograph (400x) highlighted the district from the corresponding green rectangle.
Table 1.
Colon tumor incidence, pathological characteristics, numbers in both DMH-treated and control groups.
Table 2.
Statistics of valid sequences, trimed sequences and corresponding percentage in each sample.
Figure 2.
Principal component analysis (PCA) scores plot based on the relative abundance of OTUs (97% similarity level).
Each symbol represents a sample. Red circles represent healthy rats; Blue circles represent CRC rats.
Figure 3.
Different structures of gut microbiota between healthy rats and CRC rats.
(A) Taxonomic representation of statistically and biologically consistent differences between healthy rats and CRC rats. Differences are represented by the color of the most abundant class (red indicating control group, green tumor group and yellow non-significant). The diameter of each circle’s diameter is proportional to the taxon’s abundance. (B) Histogram of the LDA scores for differentially abundant genera. Cladogram was calculated by LefSe, and displayed according to effect size.
Figure 4.
Relative contributions of dominant phyla in the intestinal lumen microbiota.
(A) healthy rats, (B) CRC rats. Histogram represents the relative abundance of bacterial phyla in microbiota of each sample (C). “Others” represents the unclassified bacteria, Actinobacteria, Cyanobacteria, Deferribacteres, Elusimicrobia, Fusobacteria and Tenericutes.
Table 3.
Relative contribution, median and range of the predominant phyla in the gut microbiota of healthy rats and CRC rats.
Figure 5.
Relative abundance of significantly different genera between healthy rats and CRC rats.
The Mann-Whitney test was used to evaluate the importance of comparisons between indicated groups.