Table 1.
List of primer pairs and starting positions within the selected markers used in the present study.
Table 2.
Primer-combinations for COI and 28S rDNA amplification.
Table 3.
List of morphologically determined species of Protura investigated in this study.
Figure 1.
NJ tree based on K2P distances from 91 COI sequences of Protura.
Newly sequenced specimens labeled with lab code number (HP), abbreviation for genus, and species name. Color code for genera: Acerentomon = violet, Ionescuellum = green, Acerentulus = orange, Acerella = red, Eosentomon = blue; Austrian sample sites are coded with different icons: Leopoldsberg = square, Eichkogel = triangle, and Twimberger Graben = circle. Bootstrap support (given below nodes) derived from 5000 replicates. Maximally supported clusters and subclusters are indicated by black dots. Genus abbreviations: Aco = Acerentomon, Ion = Ionescuellum, Acu = Acerentulus, Ace = Acerella, and Eos = Eosentomon.
Table 4.
K2P distances in COI among species of Protura collected in Austria.
Figure 2.
Comparison of COI and 28S rDNA in species discrimination of the genera Ionescuellum (Ion) and Eosentomon (Eos).
NJ tree based on K2P distances of COI (left) and the mirrored 28S rDNA results (right). Bootstrap support (maximal support marked with full circles) derived from 5000 replicates is maximal for all species and polpulations. Color code for genera: Acerentomon = violet, Ionescuellum = green, Acerentulus = orange, Acerella = red, Eosentomon = blue; Austrian sample sites are coded with different icons: Leopoldsberg = square, Eichkogel = triangle, and Twimberger Graben = circle.
Figure 3.
Comparison of COI and 28S rDNA in species discrimination of the genera Acerentomon (Aco), Acerentulus (Acu) and Acerella (Ace).
Mirrored NJ tree based on K2P distances of COI (left) and 28S (right). Bootstrap support (maximal support marked with full circles) derived from 5000 replicates. Color code for genera: Acerentomon = violet, Ionescuellum = green, Acerentulus = orange, Acerella = red, Eosentomon = blue; Austrian sample sites are coded with different icons: Leopoldsberg = square, Eichkogel = triangle, and Twimberger Graben = circle.