Table I.
Template-specific primer sequences for 454 sequencing.
Table II.
Numbers of IgE and IgG sequences in Datasets A, B and C.
Table III.
Numbers of sequences of possibly low qualitya in unfiltered (A) and filtered (B and C) datasets.
Figure 1.
Mean numbers of mutations in IGHV genes derived from IgE-associated amplified from venom allergic (V IgE), peanut allergic donors (P IgE) and IgG for Dataset A, B and C.
The exact specificities encoded by the IgE and IgG sequences are unknown.
Figure 2.
The proportion of total IGHV mutations (Mv) that are replacement mutations within the CDR1 and CDR2 regions (RCDR).
The proportions of Mv that are RCDR mutations are plotted against the total numbers of mutations in IgE sequences of unknown specificity, but derived from Venom (▪, n = 33) and Peanut (△, n = 57) allergic patients (A), and in IgG sequences (n = 411) (B). The solid lines show the 97.5% confidence limits for the RCDR/MV ratio in a model of random mutation, where p(RCDR) equals 0.26. Data points have been adjusted to highlight clusters of overlaid values, and points above the line of the random model are considered to show evidence of antigen selection.
Figure 3.
The percentages of sequences in Datasets A, B and C that showed evidence of antigen selection.