Figure 1.
Adolescent binge EtOH exposure paradigm.
Diagram depicting the experimental design. Male and female Wistar rats were purchased from Charles River Laboratories at weaning (PND 23). Animals were handled for 5 min/1x/day beginning on PND 30. Hatched box indicates treatment days for the binge EtOH exposure paradigm. Control rats were given tap water once/day during the 8-day treatment period. Rats were mated according to treatment groups on PND 75. Offspring were untreated and sacrificed at PND 7.
Table 1.
Primer sequences for selected genes.
Table 2.
Gene cluster analysis using DAVID software.
Figure 2.
Effects of parental adolescent binge EtOH exposure on the expression of hypothalamic genes involved in neurodevelopment in the hypothalamus of PND 7 male and female offspring.
qRT-PCR analysis of FGF13 (A), BMP1 (B), Reln (C) and Serpini1 (D) mRNA expression in the hypothalamus of PND 7 male and female pups from water- or binge EtOH-treated parents (white and black bars, respectively). Data are expressed as mean ± SEM of mRNA fold change in relation to control pups from water-treated parents. (*) indicates a statistically significant difference from control (p<0.05).
Figure 3.
Effects of parental adolescent binge EtOH exposure on the expression of hypothalamic genes involved in synaptic plasticity in the hypothalamus of PND 7 male and female offspring.
qRT-PCR analysis of PAK3 (A), IGF2R (B), VAMP3 (C) and FGFR3 (D) mRNA expression in the hypothalamus of PND 7 male and female pups from water- or binge EtOH - treated parents (white and black bars, respectively. Data are expressed as mean ± SEM of mRNA fold change in relation to control pups from water- treated parents. (*) indicates a statistically significant difference from control (p<0.05).
Figure 4.
Effects of parental adolescent binge EtOH exposure on the expression of hypothalamic genes involved in metabolic functions in the hypothalamus of PND 7 male and female offspring.
qRT-PCR analysis of GnRH (A), ApoE (B) mRNA expression in the hypothalamus of PND 7 male and female pups from water- or binge EtOH - treated parents (white and black bars, respectively). Data are expressed as mean ± SEM of mRNA fold change in relation to control pups from water- treated parents. (*) indicates a statistically significant difference from control (p<0.05).
Figure 5.
Effects of parental adolescent binge EtOH exposure on the expression of hypothalamic genes involved in transcription and translational regulation in the hypothalamus of PND 7 male and female offspring.
qRT-PCR analysis of Egr2 (A), DICER1 (B), Wbp4 (C) and SUMO2 (D) mRNA expression in the hypothalamus of PND 7 male and female pups from water- or binge EtOH - treated parents (white and black bars, respectively). Data are expressed as mean ± SEM of mRNA fold change in relation to control pups from water- treated parents. (*) indicates a statistically significant difference from control (p<0.05).
Figure 6.
Effects of parental adolescent binge EtOH exposure on the expression of hypothalamic genes involved in chromatin modifications in the hypothalamus of PND 7 male and female offspring.
qRT-PCR analysis of HDAC3 (A), SIRT2 (B), DNMT1 (C) mRNA expression in the hypothalamus of PND 7 male and female pups from water- or binge EtOH - treated parents (white and black bars, respectively). Data are expressed as mean ± SEM of mRNA fold change in relation to control pups from water- treated parents. (*) indicates a statistically significant difference from control (p<0.05).