Figure 1.
Lysine acetylation status is analyzed by using SDS-PAGE and Western blotting.
Protein samples were collected from different tissues: 1. Suspension cells, 2. Endosperm (5 days after anthesis), 3. Flower, and 4. Leaf. Equal amount of protein samples were loaded for western blot analysis. Proteins samples were separated on SDS-polyacrylamide gel and transferred to PVDF (Millipore) membrane. Acetylated Lys antibody (ImmuneChem) was used in a 1∶1000 dilution as previously described Finkemeier et al., 2011 [20].
Figure 2.
Representative fragmentation spectra of acetylated peptides in rice.
The detected b- and y- fragment ion series are shown. The CID fragmentation spectrum of acetylated peptide unique to (A) Dihydroorotate dihydrogenase, (B) Enolase, (C) Histone H2A, and (D) Histone H4.
Table 1.
List of lysine acetylated proteins identified in rice.
Figure 3.
Characteristics of acetylated lysine sites in rice.
(A) Amino acid frequency percentages for ±6 amino acids from lysine acetylated site. Letter K in the center represents the acetylated lysine. Amino acid frequency percentages are calculated by considering all identified lysine acetylated sites on different proteins in rice. (B) Different secondary structural regions of acetylated lysines identified in rice.
Figure 4.
Distribution of the identified lysine acetylated proteins among different Gene Ontology categories.
The pie chart was generated using the analysis results of the GOSlimViewer tool at AgBase. Percentage distribution of the unique proteins was used to make the pie charts. Gene distribution was grouped on the basis of Cellular Components (A), Molecular Functions (B) and Biological Processes (C).
Figure 5.
Comparison or subcellular distribution of lysine acetylated proteins in Arabidopsis and rice.
Cellular components of identified rice acetylated proteins were compared with the recently reported Arabidopsis acetylated proteins data [21].
Figure 6.
Diversified functions of lysine acetylation in various cellular processes in rice.
K represent lysine residue, KAT represents lysine acetyltransferase, KDAC represents lysine deacetylase.