Figure 1.
Effect of honey bee larval bodily fluid on Paenibacillus larvae growth.
Effect of different concentrations of honey bee larval bodily fluid on the in vitro growth of P. larvae bacterial cells, expressed as the optical density measured at a wavelength of 590 nm (OD590) in function of time (hours). Growth alterations were determined for six bodily fluid concentrations, expressed as the fold dilution in BHIT broth cultures: 10× dilution (♦), 25× dilution (▪), 50× dilution (▴), 100× dilution (⋄), 250× dilution (□), 500× dilution (▵), control (○). Each point in the graph displays the mean of three independent replicates, with the error bars being the standard deviations. Time 0 represents the time of spiking. Trend lines are calculated with DMfit. No OD measurements were performed during lag phase.
Figure 2.
Effect of honey bee larval bodily fluid on Paenibacillus larvae bacterial density.
OD590 (white bars) and CFU/ml (black bars) for T1, C1, T4 and C4. T1 (n = 12): test sample collected one hour after spiking with 5% larval fluids. T4 (n = 12): test sample collected four hours after spiking with 5% larval fluids. C1 (n = 11): control sample collected one hour after spiking with BHIT-broth. C4 (n = 12): control sample collected four hours after spiking with BHIT-broth. Between brackets (n): number of independent replicates. Error bars: standard deviations.
Table 1.
General overview of the microarray experiment, showing the total number of up- and down-regulated genes, for three selection criteria (columns) and four comparisons (rows).
Table 2.
GO-enrichment analysis for comparison T1–C1, showing the most specific over- and under-represented biological process GO-terms for both up- and down-regulation.
Table 3.
GO-enrichment analysis for comparison T4–C4, showing the most specific over- and under-represented biological process GO-terms for both up- and down-regulation.
Table 4.
Annotation and pathway analysis for both up- and down-regulated genes for T1–C1.
Table 5.
Annotation and pathway analysis for both up- and down-regulated genes for T4-C4.
Figure 3.
Differential expression of transporter encoding genes.
Stacked percentage bar chart, showing the numbers of up- and down-regulated (putative) transporter encoding genes for T4-C4 (left) and T1-C1 (right), respectively, relative to the total numbers of (putative) transporter encoding genes within the P. larvae genome. The latter are indicated between square brackets. Round brackets: GO term numbers. GO terms were assigned with Blast2GO. White bars: down-regulation for T4-C4. Dark grey bars: up-regulation for T4-C4. Light grey bars: down-regulation for T1-C1. Black bars: up-regulation for T1-C1. Arrow heads: arbitrary GO term hierarchy (◂>⊲><$>\raster(90%)="rg3"<$>).
Figure 4.
Differential expression of metabolic genes.
Stacked percentage bar chart, showing the numbers of up- and down-regulated (putative) metabolic genes for T4-C4 (left) and T1-C1 (right), respectively, relative to the total numbers of (putative) metabolic genes within the P. larvae genome. C: carbohydrate metabolism. The latter are indicated between square brackets. Round brackets: KO numbers (KEGG pathways). KEGG pathways were assigned with KAAS. White bars: down-regulation for T4-C4. Dark grey bars: up-regulation for T4-C4. Light grey bars: down-regulation for T1-C1. Black bars: up-regulation for T1-C1. E: energy metabolism. L: lipid metabolism. N: nucleotide metabolism. A: amino acid metabolism. ∼: KO:00061; KO:00071; KO:00592; KO:01040. *: KO:00360; KO:00350; KO:00380. °: KO:00410; KO:00430; KO:00450; KO:00460; KO:00480. ?: KO:00740; KO:00770; KO:00780; KO:00670; KO:00860; KO:00130. ‘:KO:00900; KO:00903; KO:00281; KO:00523; KO:01053; KO:01055; KO:00940; KO:00311; KO:00521. ‘’: KO:00362; KO:00627; KO:00625; KO:00622; KO:00633; KO:00642; KO:00643; KO:00930; KO:00363; KO:00621; KO:00626.
Figure 5.
Numbers of genes that are differentially expressed per subset.
Stacked percentage bar chart, showing the numbers of up- and down-regulated genes for T4-C4 (left) and T1-C1 (right), respectively, relative to the total numbers of genes within the P. larvae genome. The latter are indicated between square brackets. Round brackets: COG functional category label. C: cellular processes and signaling. I: information storage and processing. M: metabolism. P: poorly characterized. White bars: down-regulation for T4-C4. Dark grey bars: up-regulation for T4-C4. Light grey bars: down-regulation for T1-C1. Black bars: up-regulation for T1-C1.