Figure 1.
Survival of control and bre larvae developing under normoxic or hypoxic conditions from 3 dpf on (A). Survival rate of hypoxic control and bre larvae with hypoxic incubation started at different developmental stages (B). Data are shown as mean ± SE. Significance versus normoxic control is marked with *.
Figure 2.
HIF-1α protein expression of control and bre larvae developing under normoxic or hypoxic conditions. Median fold induction was calculated setting the 3 dpf normoxic control value to 1.
Figure 3.
Heart rate (A), stroke volume (B), cardiac output (C), blood cell concentration (D) and oxygen carrying capacity (E) of control and bre larvae developing under normoxic or hypoxic conditions. Data are shown as mean ± SE. Significance versus normoxic control is marked with *. Significant differences between all other groups are listed in the table.
Figure 4.
Cox 1(A) of control and bre larvae developing under normoxic or hypoxic conditions. Data are shown as mean ± SE. Significance versus normoxic control is marked with *. Cox1 DNA amount of control and bre larvae developing under normoxic or hypoxic conditions (B). Data are shown as mean ± SE. Significance versus normoxic control is marked with *. Significant differences between all other groups are listed in the table. Normalization of cox1 mRNA expression to cox1 DNA content of control and bre larvae developing under normoxic or hypoxic conditions (C). Representative images of mitochondrial staining with MitoTracker®Red (D) of normoxic control and bre animals (clipping of trunk area).
Figure 5.
AMPK protein expression of control and bre larvae developing under normoxic or hypoxic conditions presenting the percentage of pAMPK in the overall AMPK protein expression. Data are shown as mean ± SE. Significance versus normoxic control is marked with *. Significant differences between all other groups are listed in the table.
Figure 6.
Lactate accumulation of control and bre larvae developing under normoxic or hypoxic conditions. Data are shown as mean ± SE. Significance versus normoxic control is marked with *. Significant differences between all other groups are listed in the table.
Figure 7.
Lipid accumulation (green - esterified cholesterol, red – NEFAs). Total NEFAs stained are (A) lipid accumulation of Resveratrol or Troglitazone incubated control larvae (B) and of control and bre larvae developing under normoxic or hypoxic conditions.
Table 1.
Statistical analysis of NileRed staining.
Figure 8.
Total daily activity of 3 to 7bre larvae developing under normoxic or hypoxic conditions (A). Body length of control and bre larvae developing under normoxic or hypoxic conditions (B). Data are shown as mean ± SE. Significance versus normoxic control is marked with *. Significant differences between all other groups are listed in the table.
Figure 9.
pdk2 (A) and ucp1 (B) mRNA expression of control and bre larvae developing under normoxic or hypoxic conditions. Data are shown as mean ± SE. Significance versus normoxic control is marked with *.
Table 2.
Microarray analysis.