Table 1.
L. monocytogenes strains used in this study and their serotypic profiles.
Figure 1.
Multiplex PCR profiles of L. monocytogenes strains.
Lanes: MW, Molecular weight markers. Lane 1: LS1 serotype 1/2a, Lane 2: LS402, serotype 4b; Lane3-7: serotype IVb-v1 strains LS542; LS642; LS643; LS644; LS645, respectively.
Table 2.
The binary gene typing profiles of the L. monocytogenes strains.
Table 3.
The MLVA profiles of the L. monocytogenes strains.
Figure 2.
ApaI/AscI PFGE profiles of L. monocytogenes strains 4b and IVb-v1.
The dendrogram was calculated and drawn using Bionumerics software. NSW; New South Wales, VIC; Victoria, CA; California, CT; Connecticut, and NK;Not known.
Figure 3.
A neighbor-net constructed from the gene contents of 28 strains belonging to serotype 4b.
The parallel edges represent incompatible signals indicative of independent gene loss or gain due to the multiple transduction or recombination. Serotypes and epidemic clones are grouped in different color as indicated. Node labels refer to strain names (Table 1 and Laksanalamai et al 2012 (7). Scale bar represents number of gene differences (present or absent) per gene site.
Figure 4.
Hierarchical clustering based on the Robust Multi Array (RMA) analysis of the L. monocytogenes strains serotypes 4b (LS406, LS412) and IVb-v1 (LS542, LS642, LS643, LS644, LS645).
Figure 5.
Scatter plots of the summarized Robust Multi-Array Averaging (RMA) intensities.
A–C, between serotypes 4b (LS412) and IVb-v1 (LS642, A; LS644, B; LS645, C). D–E, between the same serotype, IVb-v1, isolated from Australia; from the different states (LS644 and LS645, D); from the same state (LS642 and LS644, E). F, between the same serotype, IVb-v1, isolated from the different countries (LS642; Australia and LS542; USA).
Figure 6.
The organization of the genomic region containing the lmo0737 to lmo0739 cassette that is present only in the L. monocytogenes serotypes IVb-v1 and 1/2a strains, confirmed by microarray analysis.
Table 4.
Unique probe-sets in 1/2a and IVb-v1 L. monocytogenes strains.
Table 5.
Susceptibility to Mbo1 and Sau3A digestion and distribution of the genes in the restriction-modification cassette in selected L. monocytogenes strains.