Figure 1.
A representative example of antibodies that react with both canine and murine full-length dystrophin.
Immunofluorescence staining and western blot were performed on mouse and dog muscles from normal and dystrophic animals using MANDYS1, an epitope specific antibody against exons encoding repeats 10 and 11 of the central rod domain of dystrophin. Scale bar, 50 µm.
Table 1.
A summary of selective antibodies based on their reactivity.
Table 2.
Antibodies that show cross-reactive bands in western blot.
Figure 2.
A representative example of antibodies that only react with mouse dystrophin.
Immunofluorescence staining and western blot were performed on mouse and dog muscles from normal and dystrophic animals using MANEX1216B, an epitope specific antibody against exons encoding repeat 12 through 16 of the central rod domain of dystrophin. Scale bar, 50 µm.
Figure 3.
MANEX4850D only reacts with dog dystrophin.
Immunofluorescence staining and western blot were performed on mouse and dog muscles from normal and dystrophic animals using MANEX4850D, an epitope specific antibody against exons encoding repeat 19 and hinge 3 of the central rod domain of dystrophin. Scale bar, 50 µm.
Figure 4.
A representative example of antibodies that only work for immunostaining.
Immunofluorescence staining and western blot were performed on mouse and dog muscles from normal and dystrophic animals using MANDYS111, an epitope specific antibody against exons encoding repeat 15 of the central rod domain of dystrophin. Scale bar, 50 µm.
Figure 5.
A representative example of antibodies that only work in western blot.
Immunofluorescence staining and western blot were performed on mouse and dog muscles from normal and dystrophic animals using MANDYS141, an epitope specific antibody against exons encoding repeat 14 of the central rod domain of dystrophin. Scale bar, 50 µm.
Figure 6.
MANHINGE4A is a heart specific antibody.
Immunofluorescence staining and western blot were performed on mouse and dog muscles from normal and dystrophic animals using MANDYS141, an epitope specific antibody against exons encoding hinge 4 of the central rod domain of dystrophin. Scale bar, 50 µm.
Figure 7.
A representative example of antibodies that do not recognize murine and canine dystrophin.
Immunofluorescence staining and western blot were performed on mouse and dog muscles from normal and dystrophic animals using MANEX8A. This antibody is developed to react with an epitope located in exons encoding the N-terminal domain and hinge 1. Scale bar, 50 µm.
Table 3.
Antibodies that revealed an approximately full-length size band in dystrophic muscle western blot.
Table 4.
Antibodies that we recommend for canine study (immunostaining and western blot).
Table 5.
Antibodies that we recommend for use in immunostaining only in canine study.