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Table 1.

Quantitative estimates of occurrences of different chromosomal aberrations observed in Allium cepa root tip cells after exposure to the different concentrations (12.5, 25, 50, 100 µg/mL) of TiO2 NPs dispersion and untreated/control.

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Table 2.

Mitotic Index in percentage (MI %) with the Standard Error (SE) for different exposure concentrations of TiO2 NPs where P, M, A, T stands for Prophase,Metaphase, Anaphase and Telophase as Phase Index in percentage respectively.

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Figure 1.

Various aberrant features observed upon exposure to 12.5 µg/mL (A) Chromosome break, (B) Disturbed anaphase, (C) Micronucleus formation, (D) Nuclear notch, (E) Nuclear blebbing.

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Figure 2.

Various aberrant features observed upon exposure to 100 µg/mL(A) Chromosome break, (B) Sticky chromosome, (C) Binucleate cells, (D) Laggard chromosome, (E) Clumped chromosome, (F) Diagonal anaphase.

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Figure 3.

Various aberrant features observed under fluorescence microscope upon exposure to 12.5 µg/mL(A)Sticky anaphase, (B) Diagonal anaphase, (C) Sticky metaphase, (D) Micronucleus formation, (E) Nuclear budding, (F) Binucleate cells.

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Figure 4.

Various aberrant features observed under fluorescence microscope upon exposure to 100 µg/mL(A) Multipolar anaphase, (B) Laggard chromosome, (C)Binucleate cells.

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Figure 5.

Various aberrant features observed under confocal laser scanning microscope upon exposure to 12.5 µg/mL(A) Chromosome break, (B) Chromosome bridge formation, (C) Disturbed nucleus and Nuclear notch, (D) Budding nucleus, (E) Micronucleus formation.

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Figure 6.

Various aberrant features observed under confocal laser scanning microscope upon exposure to 100 µg/mL(A) Micronucleus formation, (B) Nuclear budding.

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Figure 7.

Comet data (% tail DNA) of Allium cepa treated with different concentration of TiO2 NPs.

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Figure 8.

Internalization of TiO2 NPs into the root tips as quantified though ICP-OES.

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Figure 9.

Oxidative stress profile upon exposure to TiO2 NPs.

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