Figure 1.
Localization of ARP2 and actin in porcine oocytes during meiotic maturation.
(A) Subcellular localization of the Arp2/3 complex in porcine oocytes during meiotic maturation. An anti-ARP2 antibody was used to detect the subcellular localization of the Arp2/3 complex. ARP2 accumulated at the cortex and in the cytoplasm of MI and MII oocytes. ARP2, green; chromatin, blue. Bar = 20 µm. (B) Subcellular localization of ARP2 and actin in porcine oocytes during meiotic maturation. A similar localization pattern was observed for ARP2 and actin in porcine oocytes. ARP2, green; actin, red; chromatin, blue. (C) Subcellular localization of ARP2 and actin in porcine cumulus cells. ARP2, green; actin, red; chromatin, blue. Bar = 20 µm.
Figure 2.
CK666 treatment inhibits the nucleation of the Arp2/3 complex in porcine oocytes.
(A) The expression of ARP2 after treatment with CK666. (B) The fluorescence intensity of ARP2 decreased significantly in oocytes treated with CK666. (C) Measurement of ARP2 fluorescence intensity after CK666 treatment *, p<0.05.
Figure 3.
CK666 treatment inhibits the maturation of porcine oocytes.
(A) Cumulus cell expansion failed to occur after treatment with CK666. (B) The oocyte maturation rate decreased significantly after treatment with CK666. *, p<0.05.
Figure 4.
CK666 treatment decreases actin nucleation.
(A) An actin cap formed at the cortex of a control oocyte. There was no actin cap formation, and actin nucleation at the plasma membrane and in the cytoplasm decreased after CK666 treatment. Actin, red; chromatin, blue. Bar = 20 µm. (B) The fluorescence intensity of actin decreased significantly in oocytes cultured with CK666. (C) Measurement of actin fluorescence intensity after CK666 treatment *, p<0.05 both at the cortex and in the cytoplasm of oocytes.