Figure 1.
Translated C. victoriae A-superfamily precursor sequences.
*, Vc1.2 precursor [10] shown for comparison is in grey; Cys, yellow; Predicted signal peptides are underlined in purple and the predicted mature peptides are underlined in black, while that of Vc1.2 is underlined in grey. This color scheme is used in all subsequent figures.
Figure 2.
Translated C. victoriae I1-superfamily (A), I2-superfamily (B) and I4-superfamily (C) precursor sequences.
*, M11.2 mature peptide [24], Ep11.1 [25] precursor, ViTx precursor [26], Gla-TxX precursor [77]and the I3-superfamily (D) precursor Ca11a [60]shown for comparison.
Figure 3.
Translated C. victoriae J-superfamily (A), M-superfamily (B), M-conomarphin (C) precursor sequences.
*, pl14a [29], Tx3.2 (tx3a) [32], TxMMSK-05 [78], LtIIIA [33], conomarphin [79], Mr038 [34] precursors shown for comparison.
Figure 4.
Translated C. victoriae O1-superfamily precursor sequences.
*, Vc6.1, Vc6.3, Vc6.4, Vc6.6 [11] and MrVIB [80] shown for comparison.
Figure 5.
Translated C. victoriae O2-superfamily (A) and contryphan (B) precursor sequences.
*, TxVIIA [78], [81] and contryphan-R/Tx precursors [82] shown for comparison.
Figure 6.
Translated C. victoriae O3-superfamily (A), P-superfamily (B) and S-superfamily (C) precursor sequences.
*, Bromosleeper peptide (GenBank: GQ981406.1) [41], Gm9.1 (GmIXA) [43], Tx8.1 [46], GVIIIA [44] and RVIIIA [45] precursors shown for comparison.
Figure 7.
Translated C. victoriae T-superfamily precursor sequences.
*, Vc5.4 [11], TxVA [78], TxXIIIA [47], and χ-MrIA [83] precursors shown for comparison.
Figure 8.
Translated C. victoriae conantokin (A), con-ikot-ikot (B), conodipine (C) and B2-superfamily (D) precursor sequences.
*, Con-Gm [51], G56 [5], con-ikot-ikot [52], p21a [53], Conodipine-M [54] and B2-superfamily sequences from C. literratus [57] and C. consors [56] are shown for comparison. The conodipine catalytic His-Asp dyad is boxed in red.
Figure 9.
Translated C. victoriae E-superfamily (A), F-superfamily (B), H-superfamily (C) and U-superfamily (D) precursor sequences.
*, Mr104 [34], Mr105 [34], other H-superfamily precursors (Mr097, Mr098, Mr099 and Mr100) [34]and the textile convulsant peptide [63] are shown for comparison.
Figure 10.
C. victoriae Hhe53-like open reading frame displaying translation of forward frames 1 and 2.
Possible initiator codon in frame 2 is underlined in purple and the sequence encoding the predicted mature peptide in frame 1 is underlined in black.
Figure 11.
Relative abundance of conotoxin superfamilies (total reads assembled for conotoxin precursors of each superfamily).
High abundance reads may be under-represented as a result of cDNA library normalization.
Table 1.
Functional diversity encoded in the venom gland transcriptome of C. victoriae.