Figure 1.
Growth curves for M. tuberculosis grown in medium containing Tween 80 as the primary carbon source.
Total viable counts for Culture 1 (circles), Culture 2 (diamonds), and Culture 3 (squares), were measured over an extended period of at least 200 days. An estimation of each growth phase is indicated on the graph: exponential phase (E), stationary phase (S), late stationary phase (LS), death phase (D), and non-replicating persistent phase (NRP).
Figure 2.
Scanning electron micrograph images of formaldehyde-fixed cells sampled from Culture 2.
Panel A) exponential phase at day 8; Panel B) stationary phase at day 44; Panel C) late stationary phase at day 107; and Panel D) NRP at day 292.
Figure 3.
Cells sampled during late stationary phase. Bacilli stained with Ziehl Neelson and the surrounding extracellular material was stained with Alcian Blue, pH×100.
Figure 4.
1D TLC of freely extractable lipids from Culture 2.
Cell biomass was sampled during exponential phase (day 8), stationary phase (day 44), late stationary phase (day 107) and non-replicating persistent phase (day 292). Lipids migrating on the TLC were visualised by staining with MPA and compared to known standards [14].
Figure 5.
2D TLC analyses of apolar lipids extracted from Culture 2 and Culture 3.
Panel A, cell biomass was sampled from culture 2 during exponential phase (day 8), stationary phase (day 44), late stationary phase (day 107), non-replicating persistent phase (day 292). Panel B, cell biomass was sampled from culture 3 during exponential phase (day 4), stationary phase (day 29), late stationary phase (day 72), NRP phase (day 277). Lipids migrating on the TLC (using solvent system C) were visualised by staining with MPA and compared to known standards [14].
Figure 6.
SDS-PAGE analysis of polysaccharide material isolated from the culture medium during the four phases of growth in culture 2 and culture 3.
Culture 2 in Panel A; polysaccharide material was purified from liquid medium prior to inoculation in lane 1, exponential phase growth (day 8) in lane 2, stationary phase growth (day 44) in lane 3, late stationary phase growth (day 107) in lane 4 and NRP phase growth (day 292) in lane 5. Culture 3 in Panel B, polysaccharide material was purified from liquid medium prior to inoculation in lane 1, exponential phase growth (day 4) in lane 2, stationary phase growth (day 29) in lane 3, late stationary phase growth (day 72) in lane 4 and NRP phase growth (day 278) lane 5. For comparison, the panel on the right shows LM (lane 1) and LAM (lane 2) previously isolated from M. tuberculosis H37Rv cultured exponentially in liquid medium.
Figure 7.
Gene expression clusters with a marked enrichment in fatty acid metabolism, lipid degradation, and cell wall re-modeling.
Graphs show normalised log expression values (centered around a mean value of zero) over the time-courses in Cultures 1 and 2. The shaded area represents the 99% confidence interval for the curves.
Table 1.
Annotation of clusters that were enriched for functional category.
Figure 8.
Gene expression profiles for LAM biosynthetic genes, Rv3257c (pmmA), Rv3793 (embC), Rv3806c (ubiA).
The lines on the graphs show the average normalised log ratios of gene expression over the time-courses in Cultures 1 and 2.
Table 2.
Genes up-regulated in late stationary phase and during chronic infection in mice.
Table 3.
Genes up-regulated in stationary phase in Cultures 1 and 2 and in further conditions in vitro or in vivo.
Figure 9.
Bacterial load at day 16 and day 42 in guinea pigs post aerosol challenge with either log phase culture or NRP culture.
Lungs in Panel A) and spleen in Panel B), challenged with either exponentially growing culture (white) or NRP culture of M. tuberculosis (black). Bars indicate group mean log10 cfu mL−1 +/− S.E.M of 8 guinea pigs. Statistical analysis was performed using paired T-tests. *** indicates P = <0.001.
Figure 10.
Histopathological changes in guinea pig lung following aerosol challenge with M. tuberculosis cultured from different growth phases of nutrient starvation at days 16 and 42 post challenge.
H&E. Magnification bar, 500 µm.
Figure 11.
Subjective histopathology scores of post mortem at 16 and 42 days post aerosol challenge.
Lung in Panel A) and spleen in Panel B) challenged with either exponentially growing culture (white) or NRP cultures of M. tuberculosis (black). Bars indicate group mean of 8 animals. Histopathological changes in the lung were recorded either as consolidated (no-shaded bars), necrotic (heavy-shaded bars) or calcified (light shaded bars). Spleen scores for each animal comprise number, size, and foci of necrosis and calcification.