Figure 1.
Thirty two healthy young men were included in the study, Where Plasma samples from 16 subject passed the first quality control for hemolysis, plasma samples from thirteen subjects passed the other. Seven plasma samples 12 weeks endurance training intervention passed the second control. A global miRNA PCR screening was performed to identify ci-miRNA expression levels (742 miNRAs). Based in on the global screening a customised PCR arrays including 188 miRNA primers were designed. The PCR data was analysed using the GenEx analysis tool where 160 miRNAs were detectable.
Table 1.
Subject characteristics.
Figure 2.
Alterations in ci-miRNAs in plasma immediately after an acute exercise bout.
Eight ci-miRNAs were down regulated after a single 60 min aerobic exercise bout at 65% Pmax. Ci-miRNAs below the Dunn-bonferroni corrected p-value P<0.00032 was considered as significant altered.
Figure 3.
Alterations in resting ci-miRNAs in plasma one hour after an acute exercise bout.
Five ci-miRNAs were up regulated 1 hour after a 60 min aerobic exercise bout at 65% Pmax when Dunn-bonferroni correction of the significance level 0.05 was performed (P<0.00032). Additional two miRNAs were borderline significant (BS) up regulated at significance level 0.001.
Table 2.
CT values are presented as mean ± SD.
Figure 4.
Alterations in ci-miRNAs in plasma three hours after an acute exercise bout.
One ci-miRNA was up regulated 3 hour after a 60 min aerobic exercise bout at 65% Pmax when Dunn-bonferroni correction of the significance level 0.05 was performed (P<0.00032). Additional three miRNAs were borderline significant (BS) up regulated at significance level 0.001.
Figure 5.
Alterations in ci-miRNAs in plasma in response to 12 weeks of endurance training.
Two ci-miRNAs were up regulated and seven were down regulated in response to 12 weeks endurance training when Dunn-bonferroni correction of the significance level 0.05 was performed (P<0.00032). Additional four miRNAs were borderline significant (BS) down regulated at significance level 0.001.