Figure 1.
Coomassie stained SDS-PAGE for MVMp-VLP (a.1), MVMp-Empty (b.1), MVMp-Full (c.1), MVMi-VLP (d.1), MVMi-Empty (e.1), MVMi-Full (f.1). The positions of low-molecular-weight (LMW) standards (in kDa; Bio-Rad, Hercules, CA, USA) are indicated on the left-hand side. Negatively stained electron micrographs are shown in (a.2), (b.2), (c.2), (d.2), (e.2), and (f.2) respectively. Images in (b.2) and (c.2) were collected at 60,000X magnification while images in (a.2), (d.2), (e.2), and (f.2) were collected at 100,000X magnification.
Figure 2.
Sialylated derivatives recognized by the MVM viruses.
The glycan number and the schematic representation are shown.
Figure 3.
SGM array data for the MVM capsids.
(A) MVMp-Full, (B) MVMp-Empty, (C) MVMp-VLP, (D) MVMi-Full, (E) MVMi-Empty, and (F) MVMi-VLP. The bars represent relative fluorescence for a given glycan. The glycans showing specificity to the MVM viruses are labeled.
Figure 4.
MALDI-TOF MS profiles of the permethylated low mass N-glycans.
Low mass N-linked glycan expression on (A) A9 cells, (B) EL4 T cells, and (C) NB324K cells is shown. The glycans are annotated in the cartoon form. Inset depicts the cartoon legend.
Figure 5.
MALDI-TOF MS profiles of the permethylated medium mass N-glycans.
Medium mass N-linked glycan expression on (A) A9 cells, (B) EL4 T cells, and (C) NB324K cells is shown. The glycans are annotated in the cartoon form. Inset depicts the cartoon legend.
Figure 6.
MALDI-TOF MS profiles of polar glycolipids.
(A) A9 cells, (B) EL4 T cells, and (C) NB324K cells. The glycans are annotated in the cartoon form. The glycolipids were deutero-reduced (DR) to differentiate between symmetrical molecules in subsequent MS/MS analyses. M and m refer to major and minor species, respectively. Inset depicts the cartoon legend.
Figure 7.
MALDI-TOF MS profiles of the O-glycans.
(A) A9 cells, (B) EL4 T cells, and (C) NB324K cells. The glycans are annotated in the cartoon form. Inset depicts the cartoon legend.