Table 1.
Kinetic constants of ABTS oxidation by solution phase and agarose encapsulated HRP in buffer.
Figure 1.
Functional stability of HRP encapsulated in agarose gels.
Percent activity retained for solution phase HRP (white bars) and HRP encapsulated in 0.5% agarose (gray bars) and 2% agarose (black bars) in the presence of 35% ethanol and isopropanol. The relative activities were calculated by normalizing the activity in the presence of denaturants to the activity in buffer. Error bars indicate the standard deviation of triplicate measurements.
Figure 2.
Kinetic constants of HRP catalysis in presence of denaturants.
Relative kinetic constants of ABTS oxidation by solution phase HRP (white bars) and HRP encapsulated in 0.5% agarose (gray bars) and 2% agarose (black bars) in the presence of 35% ethanol and isopropanol – (a) Vmax and (b) Vmax/KM. The relative kinetic constants were calculated by normalizing the kinetic parameters of HRP in the presence of denaturants to those in buffer. Error bars indicate the standard deviation of triplicate measurements.
Table 2.
Kinetic constants of ABTS oxidation by solution phase and agarose encapsulated HRP in 35% ethanol.
Figure 3.
Structural stability of enzymes encapsulated in agarose gels.
ANS fluorescence intensities (in arbitrary units) for solution phase enzyme (white) and enzyme encapsulated in 0.5% agarose (gray) and 2% agarose (black) exposed to 35% ethanol – (a) HRP and (b) β-gal. Fluorescence intensities for the agarose-protein formulations are reported after subtraction of the spectra of the corresponding agarose gels containing no enzyme.
Figure 4.
Structural stability of HRP encapsulated in agarose-alginate hybrid gels.
ANS fluorescence intensities (in arbitrary units) for HRP encapsulated in agarose-alginate hybrid gels exposed to 35% ethanol – (a) 0.5% agarose (gray) and (b) 2% agarose (black) containing 0% (circles), 0.25% (squares), or 0.5% (triangles) alginate. Fluorescence intensities for the agarose-alginate-protein formulations are reported after subtraction of the spectra of the corresponding agarose-alginate gels containing no enzyme.