Figure 1.
1H NMR spectra of the VPS before and after treatment with enzymes (50°C).
Figure 2.
Structures of the VPS-PS and its derivative.
Figure 3.
1H-13C HSQC spectrum of the VPS-PS with 1H NMR trace.
Figure 4.
GC-MS profiles of acetylated methanolysis products selected for m/z 316 (oxonium ion from hexosaminouronic acid) of VPS-PS, ECA (ManNAcA), and O-PS from P. aeruginosa PA14 (GalNAcA).
Figure 5.
Mass spectra of acetylated methyl glycosides methyl esters of 2-aminouronic acids obtained by methanolysis from VPS-PS and ECA (contains ManNAcA).
Table 1.
1H and 13C chemical shifts of the VPS-PS and its derivatives.
Figure 6.
1H NMR spectrum of the O-deacetylated VPS-PS.
Figure 7.
Positive mode MS-MS spectra of the VPS-PS (top) and O-deacylated VPS-PS (bottom).