Figure 1.
Morphology if 10-day old healthy (A), and clubroot-infected (B), and 20 day old uninfected (C), and clubroot infected (D) B. napus roots showing gall formation in the latter (open arrows) due to P. brassicae infection.
Histopathological analysis indicates the presence of evacuated zoosporangia (open arrow) in the root hair and primary plasmodia (closed arrows) in epidermal cells indicating infection (F) compared to uninoculated controls (E). At 20 dpi, the cortical cells of infected tissue show the presence of numerous secondary plasmodium (arrowheads) in the cortical cells (H) compared to the control (G). Bars represent 5 mm for Figures. A–D and 100 µm for Figures. E–H.
Figure 2.
miRNA-microarray expression of P. brassicae responsive miRNAs exhibiting differential expression at 10- (A) and 20- dpi (B, C) following pathogen infection.
Table 1.
List of miRNAs that exhibited modulation in their expression following infection by P. brassicae, their possible targets, and their annotated biological functions.
Table 2.
Possible interactions between identified miRNAs showing modulation and their predicted targets as obtained through starBase Degradome analysis.
Figure 3.
Relative abundance of miRNA in B. napus plant infected with P. brassicae.
(a) Relative accumulation of miRNA showing the quantitative expression at 10 dpi and (b) Relative accumulation of miRNA showing the quantitative expression at 20 dpi. The expression of sonR66 was used as internal control in the experiment. The error bars show the standard deviation.
Figure 4.
miRNA mapping and cleavage site determination through 5′ RLM RACE.
Agarose gel image of 5′ RACE products (A) and the target mRNA cleavage sites (B). The targeted mRNA section and miRNA sequences, along with mismatch (es), if any, are shown as the expanded region. The 5′ends of the cleaved product determined by sequencing is indicated by the vertical arrowheads, along with the numbers of clones analyzed. The horizontal arrowheads indicated the gene-specific primer sites used for 5′RLM-RACE.