Table 1.
Function, primer sequence and amplicon characteristics of the candidate reference genes used in this study.
Figure 1.
Expression levels of candidate reference genes.
The expression level of candidate N. lugens reference genes in the total samples is shown in terms of the cycle threshold number (Ct-value). The data are expressed as whisker box plots; the box represents the 25th–75th percentiles, the median is indicated by a bar across the box, the whiskers on each box represent the minimum and maximum values.
Figure 2.
Determination of the optimal number of reference genes for accurate normalization calculated by geNorm.
The value of Vn/Vn+1 indicates the pairwise variation (Y axis) between two sequential normalization factors and determines the optimal number of reference genes required for accurate normalization. A value below 0.15 indicates that an additional reference gene will not significantly improve normalization.
Table 2.
Ranking order of the candidate reference genes of N. lugens in different experimental conditions.