Table 1.
miRNAs significantly altered in schizophrenia PFC.
Table 2.
miRNAs significantly altered in bipolar disorder and major depression PFC.
Table 3.
miRNAs altered by 40% or more in one or more diagnostic groups in PFC.
Figure 1.
Manual RT-PCR measurements of 3 selected miRNAs agree very well with measurements performed by TLDA assay.
The control and schizophrenia samples of Experiment 1 were measured using individual TaqMan primer sets for miR-17, miR-145-5p and miR-219-2-3p. Note that lower Ct values indicate higher abundance on a log2 scale (i.e., a decrease of 0.5 Ct represents a 41% increase in abundance or 1.41-fold difference across groups). Error bars indicate standard error of the mean. In this assay, miR-17-5p showed a 1.18-fold increase (p = 0.05), which is very close to 1.22-fold reported in Table 1. miR-145-5p showed a 0.85-fold change (p = 0.16), similar to 0.68-fold in Table 1. miR-219-2-3p showed a 1.60-fold increase (p = 0.029), similar to 1.48-fold in Table 1.
Table 4.
miRNAs altered in suicide.
Table 5.
miR-577 variants vary in 5′-end and male/female expression ratio.
Figure 2.
Human PFC sample processed for synaptosome isolation and examined for protein and total RNA.
(A) Equal amount of proteins (12 µg) were subjected to PAGE and blotted with anti-PSD95 (Millipore mouse monoclonal clone 28/43; 1∶30,000), anti-Synapsin I (Chemicon AB1543P, lot #LV1354224, rabbit polyclonal affinity purified; 1∶30,000), or anti-PCNA (Sigma, P-8825, clone PC10, mouse monoclonal; 1∶1000) antibody. Fractions are: T, total homogenate; S2, 20,000×20 min supernatant; Syn, synaptosomes. The raw data are shown in File S5. (B) Synaptosomal RNA (0.5 µg) was loaded in a 0.9% TBE Agarose gel and stained with Ethidium Bromide. MW is the 1 Kb plus ladder size markers.
Table 6.
Distribution of sequence counts by length in control vs. schizophrenia synaptosomes.
Table 7.
SNORD85-derived small RNAs that are down-regulated in schizophrenia synaptosomes.
Table 8.
Non-miRNA small RNAs that are altered in schizophrenia synaptosomes.