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Figure 1.

Rate of BRAF-V600 mutations in patients with tumor thickness of 1 mm or less (grey bars) or more than 1 mm (black bars) according to age (left), histological subtype (middle), and mitotic rate (right).

SSM – superficial spreading melanoma; NM – nodular melanoma; LMM – lentigo maligna melanoma; ALM – acral lentiginous melanoma.

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Table 1.

Association of BRAF mutation status with clinicopathological parameters.

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Table 2.

Association of BRAF mutational status with clinicopathological parameters stratified according to tumor thickness.

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Figure 2.

Univariate survival analysis according to BRAF-V600 mutational status.

No differences in overall survival (A) but a trend for unfavorable distant metastases-free survival (B) was observed in patients with tumor BRAF mutations. Survival after occurrence of distant metastasis was not different according to the tumor BRAF mutational status (C).

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Table 3.

Analysis of overall survival.

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Table 4.

Overall survival stratified according to tumor thickness.

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Figure 3.

Kaplan-Meier analysis of overall survival (OS) of patients stratified according to tumor thickness for BRAF-V600 mutant (BRAF-mut.) vs. wild-type (WT) patients with tumor thickness ≤1 mm (A) or with tumor thickness >1 mm (B).

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Figure 4.

Rate of BRAF-V600 tumor mutations according to disease outcome.

A significantly lower rate of BRAF-V600 tumor mutations was observed in 382 patients who did not develop distant metastasis during follow-up (upper black solid bar - w/o distant metastasis) compared to 55 stage I/II patients who had distant recurrences in the further course of disease (black solid bar - with distant metastases) in our study (36.6% versus 52.7%; p = 0.026). A similar difference in mutational rate was reported in prior studies comparing the mutational rate in metastases of late-stage melanoma patients and primary tumors of stage I/II patients.

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