Figure 1.
Effect of LPS and ATP treatment on the pyroptosis of J774 cells.
Macrophage-like J774 cells were primed with 10 ng/ml LPS for 4 h, and then treated with 3 mM ATP for the indicated time periods in the absence or presence of 20 µM Ac-YVAD-CHO, a caspase-1 specific inhibitor. Cells were also incubated with LPS or ATP alone, or without LPS and ATP (Resting). Thereafter, the supernatants were recovered for the assays of IL-1β (A) and LDH (B). IL-1β 1evels were determined using a commercially available mouse IL-1β ELISA kit. LDH activities in the supernatants and 1% Triton X-100-lysed cells (as a total activity of 100%) were determined using a commercially available LDH assay kit. Data shows the mean ± SD of 3 separate experiments. Values are compared between the absence and presence of Ac-YVAD-CHO. **P<0.01, ***P< 0.001. (C) J774 cells were primed with 10 ng/ml LPS for 4 h and then treated with 3 mM ATP for 90 min. Thereafter, the cells were stained with FAM-YVAD-fmk (a fluorescent labeled caspase-1 inhibitor for inflammasome staining, green) and Hoechst 33342 (for nuclear staining, blue) and photographed with a fluorescence microscope system. Arrowheads indicate the inflammasomes containing the activated caspase-1. Images of cells are representative of 3 separate experiments.
Figure 2.
Effect of LL-37 on the LPS/ATP-induced pyroptosis of J774 cells.
J774 cells were primed with 10/ml LPS for 4 h, and then treated with 3 mM ATP for 90 min in the absence or presence of LL-37 (0.01, 0.1 or 1 µg/ml). Cells were also incubated with LPS or ATP alone, or without LPS and ATP. Thereafter, the supernatants were recovered for the assays of IL-1β (A) and LDH (C), and the cells were used for the assays of IL-1β mRNA expression (B) and caspase-1 activation (D). IL-1β mRNA expression were determined by RT-PCR and expressed as fold increase relative to resting cells incubated without LPS, ATP and LL-37; the caspase-1 activation was assayed by flow cytometry using FAM-YVAD-fmk (a fluorescent labeled inhibitor of caspase-1, FLICA) that irreversibly binds with activated caspase-1, and expressed as the percentage of FLICA positive cells. Data shows the mean ± SD of 3-5 separate experiments. Values are compared between the absence and presence of LL-37 among LPS/ATP-treated cells. *P<0.05, **P<0.01, ***P<0.001. Images of RT-PCR are representative of 3-5 separate experiments.
Figure 3.
Effect of LL-37 on the LPS/ATP-induced inflammasome formation in J774 cells.
J774 cells were primed with 10/ml LPS for 4 h, and then treated with 3 mM ATP for 90 min in the absence or presence of LL-37 (1 µg/ml). Cells were also incubated with LPS or ATP alone, or without LPS and ATP (Resting). Thereafter, the cells were stained with FAM-YVAD-fmk (a fluorescent labeled caspase-1 inhibitor) and Hoechst 33342 (A). Arrowheads indicate the inflammasomes containing the activated caspase-1. Furthermore, the percentage of inflammasome-containing cells with activated caspase-1 was determined by counting at least 200 Hoechst positive cells (B). Data shows the mean ± SD of 3-5 separate experiments. Values are compared in the LPS/ATP-treated cells between the absence and presence of LL-37. ***P<0.001. Images cells are representative of 3–5 separate experiments.
Figure 4.
Effect of LL-37 on the LPS binding to J774 cells.
J774 cells were suspended in DMEM containing 10% FBS, and incubated with 1 µg/ml FITC-LPS at 37°C for 15 min in the absence or presence of LL-37 (0.01, 0.1 or 1 µg/ml), anti-mouse CD14 monoclonal antibody (4C1, 10 µg/ml), anti-mouse TLR4 monoclonal antibody (MTS510, 40 µg/ml) or isotype control IgG (IgG2b and IgG2a). The binding of LPS was analyzed by flow cytometry, and the median fluorescence intensity was determined. The LPS binding was expressed as the percentage of that with FITC-LPS alone. Data shows the mean ± SD of 3 separate experiments. Values are compared between the absence and presence of LL-37, anti-CD14 monoclonal antibody or anti-TLR4 monoclonal antibody. **P<0.01, ***P<0.001.
Figure 5.
Effect of LL-37 on the ATP-induced caspase-1 activation in J774 cells.
J774 cells were treated with 3-37 (0.01, 0.1 or 1 µg/ml), P2X7 antagonists (1 µM KN-62 and 1 µM KN-93) or dimethylsulfoxide (DMSO, a solvent for KN-62 and KN-93, 0.1%). Cells were also incubated without ATP, LL-37 and P2X7 antagonists (Resting). Thereafter, the caspase-1 activation was assayed by flow cytometry using FAM-YVAD-fmk (FLICA), and expressed as the percentage of FLICA positive cells. Data shows the mean ± SD of 3 separate experiments. Values are compared between the absence and presence of LL-37 or P2X7 antagonists among ATP-treated cells. **P<0.01, ***P<0.001.