Table 1.
Patient and Lesion Characteristics for Stool Study.
Figure 1.
Tissue levels of aberrantly methylated genes.
Tissue levels of methylated BMP3 (mBMP3) and NDRG4 (mNDRG4) genes are compared in normal colorectal mucosa, n = 20 unique control patients, and sessile serrated polyps (SSP), n = 20 unique case patients. Marker levels are normalized to β-actin (a marker of total human DNA) and expressed in relative units. Levels were substantially and significantly higher in SSP than normal colon for both mBMP3 (p<0.0001) and mNDRG4 (p<0.0001).
Figure 2.
Detection of SSP by stool assay of mBMP3 and by fecal immunochemical testing (FIT).
FIT sensitivity was evaluated at the conventional cutoff of 100/ml buffer (FIT-100) and at 50 ng/ml (FIT-50). Specificity cutoffs for stool DNA marker mBMP3 were selected to match those for both FIT-100 and FIT-50 so that sensitivities could be most meaningfully compared.