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Table 1.

Sample list and accession numbers.

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Figure 1.

Time course of CDV outbreak in Denmark 2011–2013.

The diagram illustrates the outline of the CDV outbreak based on number of positive samples investigated at NVI during 2011–2013 on monthly basis and divided in mink, fox, and other wildlife samples.

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Table 2.

List of primers used for canine distemper virus H-gene RT-PCR and sequencing.

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Figure 2.

Phylogenetic tree of the CDV H-gene.

Phylogenetic tree based on nucleotide sequences of the H-gene (position 1–1824) from outbreak canine distemper viruses and reference viruses. Phylogenetic trees were constructed using distance-based method with the Neighbor Joining algorithm and bootstrap analysis with 1000 replicates.

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Figure 3.

Geographical spread of the nucleotide sequence groupings of the CDV H-gene.

Map of Jutland with geographical localization of CDV groupings based on the nucleotide sequence of the H-gene. The symbols represent the following substitutions: circle: C543T; Square: G726T, 1630A, 1814C; Rhomboid: A740C, 1630G, 1814T; Star: T1057C, C1340A, 1630A, 1814C; Upright triangle: T1215C, 1620A, 1814C; Downright triangle: A1304G, 1630A, 1814C; Cross: C1340A, 1630A; Snowflake: C543T, A1412G, 1630G, 1814T; Flag: 1630A, T1645C, 1814C; Open circle with rhomboid: virus sequences not classified into the above mentioned groups.

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Table 3.

Nucleotide differences in the H-gene of outbreak viruses.

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Table 3 Expand

Table 4.

Amino acid differences in the H-protein of the outbreak viruses.

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