Figure 1.
Steady increment of IPF and A-IPC in SDP during storage.
A, Platelet count, %-IPF and A-IPC of different SDP on day 0, 3, 5 and 7 post donation during storage; N represents the number of SDP specimens measured experimentally. B–D, Serial monitoring of platelet parameters in SDP during storage including platelet count (B), %-IPF (C) and A-IPC (D) on day 3, 5, 7, 10, and 14 (N = 5). * p<0.05, ** p<0.005, *** p<0.001 when compared to day 3. Both Mean and SEM are reported for each specimen per time point.
Figure 2.
A–C, Serial monitoring of platelet parameters in SDP during storage (A, %-H-IPF; B, P-LCR; C, MPV, N = 5).
* p<0.05, ** p<0.005, *** p<0.001 when compared to day 3. Both Mean and SEM are reported for each specimen per time point. D, Platelet parameters on day 5 post donation in response to gamma-irradiation; N = 6/group.
Figure 3.
A–D, Transmission Electron Microscopy (TEM) analysis of platelet morphology in SDP units during prolonged storage.
A, Representative image of day 3 platelets and immature platelets in SDP; scale bar indicates 2 µm. B, Cross-sectional image of the immature platelet on day 3 as shown in Fig 3A with an adjacent erythrocyte for size comparison. The immature platelets contains glycogen, mitochondria, dense granules, alpha granules, and open canalicular system; scale bar indicates 500 nm. C, Representative image of the cross-sectional image of platelet in SDP on day 10; scale bar indicates 2 µm. D, Cross-sectional image of the immature platelet on day 10 as shown in Fig 3C; scale bar indicates 500 nm.