Figure 1.
Effect of carboplatin and paclitaxel treatment on A2780 tumor growth.
Mice implanted with A2780 xenograft tumors were treated with a combination of carboplatin (40 mg/kg ip) and paclitaxel (10 mg/kg iv) on day 0 and 5. Tumor sizes in the carboplatin and paclitaxel group (n=14 tumors) were significantly different than in the control group (n=12 tumors) on day 8 (p=0.034). The tumor sizes were measured with microCT and presented as mean±SEM.
Figure 2.
FDG uptakes after treatment with carboplatin and paclitaxel.
FDG uptake was analyzed one hour post injection in a carboplatin/paclitaxel treatment (n=8 tumors) and a control group (n=5 tumors). The mice were PET/CT scanned at baseline before treatment start and day 1, 4 and 8 after injection of first dose. A) Tumor uptake of FDG during treatment of A2780 xenograft tumors with carboplatin and paclitaxel. Quantitative tumor uptake is presented as SUVmean and SUVmax (mean±SEM). B) Representative PET/CT images of one mouse from the carboplatin and paclitaxel treatment group (upper images) and one mouse from the control vehicle treated group (lower images). Dotted circles indicate the tumors.
Figure 3.
FLT uptakes after treatment with carboplatin and paclitaxel.
FLT uptake was analyzed one hour post injection in a carboplatin/paclitaxel treatment (n=6 tumors) and a control group (n=7 tumors). The mice were PET/CT scanned at baseline before treatment start and day 1, 4 and 8 after injection of first dose. A) Tumor uptake of FLT during treatment of A2780 xenograft tumors with carboplatin and paclitaxel. Quantitative tumor uptake is presented as SUVmean and SUVmax (mean±SEM). B) Representative PET/CT images of one mouse from the carboplatin and paclitaxel treatment group (upper images) and one mouse from the control vehicle treated group (lower images). On the images of the mouse from the treatment group two tumors are visible whereas on the images of the control mouse only one tumor is visible. Dotted circles indicate the tumors.
Figure 4.
Gene expression of GLUT1, HK1, HK2, Ki67 and TK1.
On day 8 immediately after the last PET/CT scan all tumors were excised and total RNA was isolated and afterward revers transcribed into cDNA. With qPCR relative expression of GLUT1, HK1, HK2, Ki67 and TK1 were measured. The levels of the gene of interests were normalized to the geometric means of two reference genes GUSB and HPRT. A) For the FDG study GLUT1 expression was decreased, the difference not being statistically significant (p=0.08), HK1 expression was lower in the treatment compared to the control group (p=0.03). B) For the FLT study no difference in Ki67 and TK1 between the treatment and control group was observed on day 8. The data are presented as mean±SEM.