Table 1.
Clinicopathological demographics of 190 Chinese prostate cancer patients.
Figure 1.
FISH probe design and representative images of ERG rearrangement.
(A) Schematic map of ‘TMPRSS2’ and ‘ERG’ position on 21q22.2–22.3. T and C orientate toward the telomeric and centromeric regions, respectively. BACs located 5′ and 3′ to ERG were used as probes for interphase FISH. Chromosomal coordinates are from the March 2006 build of the human genome using the UCSC Genome Browser. The TMPRSS2and ERG loci are separated by approximately 3 Mb. (B) FISH was performed using BACs as indicated with the corresponding fluorescent label on formalin-fixed paraffin-embedded tissue sections for break-apart FISH of the ERG gene. (B & E), ERG rearrangement negative case, as indicated by two pairs of co-localized green and red signals. (C & F), ERG rearrangement positive (translocation) case showed one pair of split 5′ and 3′ signals. (D & G), ERG rearrangement positive (with deletion) case showed loss of one green labeled probe 5′ to ERG.
Figure 2.
Representative images for IHC staining and FISH analysis of PTEN, HER2 and Ki-67 expression in PCa.
(A1–A3) FISH images of undeleted, hemizygous and homozygous PTEN deletion in PCa. A1, PTEN deletion negative case showed both paired red signals (10q23/PTEN locus) and green signals in tumor cells. A2, Representative case with PTEN hemizygous deletion showed one red signals and pairs of green signals in tumor cells. A3, Representative case with PTEN homozygous deletion showed absence of red signals but retained pairs of green signals. For all assays, at least 50 cancer cell nuclei were evaluated. (B1–B3) The detection of HER2 expression by IHC and FISH in PCa. B1, FISH analysis of representative case without HER2 amplification. B2, FISH analysis of case with HER2 amplification. B3, HER2 IHC staining shows complete membranous reactivity of strong intensity (3+) in tumor cells (original magnification, ×200). (C1–C3) The Ki-67 staining by IHC in PCa cells. C1, No staining (0) of Ki-67 in tumor cells. C2, Low Ki-67 (LI<10%) nuclear positivity in tumor cells. C3, High Ki-67(LI≥10%) nuclear positivity in tumor cells.
Table 2.
Association of clinicopathologic variables and molecular biomarkers with ERG rearrangement.
Figure 3.
Kaplan-Meier survival curves for PCa patients with and without ERG rearrangement.
The cancer-related survival rates were compared between patients with and without ERG rearrangement using the log-rank test.
Table 3.
Univariate and multivariate analysis of variables associated with survival in PCa patients.
Figure 4.
Kaplan-Meier curves illustrating cancer related survival among PCa patients.
The patients were stratified by ERG rearrangement and Ki-67 LI in combination and log-rank test was performed.
Figure 5.
Kaplan-Meier survival analysis of PCa patients in relation to ERG rearrangement status.
(A) low ki-67 LI (<10%) subgroup, (B) high ki-67 LI (≥10%) subgroup.