Figure 1.
Structures of lincosamides (A–C) and other natural compounds containing branched L-proline precursors (D–G).
(A) Lincomycin A, (B) Lincomycin B, (C) Celesticetin, (D) Anthramycin, (E) Sibiromycin, (F) Tomaymycin, (G) Hormaomycin. Fragments derived from L-proline are highlighted.
Figure 2.
Phylogenetic relationships of NRPS A-domains specific for L-proline or its derivatives.
A rooted, neighbor-joining phylogenetic tree was constructed based on the full length amino acid sequences of stand-alone A-domains and excised sequences of modular A-domains. Bootstrap values (100 replicates) above 50% are indicated at the nodes. Modular A-domains are represented by name of respective NRPS module and marked with a ■, stand-alone A-domains are marked with a ▲. The substrates for each domain include L-proline (Pro), L-proline derivatives with one carbon side chains (Pro1C), L-proline derivatives with two carbon side chains (Pro2C), and L-proline derivatives with three carbon side chain (Pro3C). Those A-domains specific for Pro2C or Pro3C substrates are highlighted. Number in parentheses behind the name of respective NRPS denotes the number of the module in NRPS protein chain, if relevant; letter in parentheses denotes the source organism. The GenBank accession numbers, producing strains, and references describing each A-domain are listed. Percent sequence identities with LmbC and CcbC were calculated from pairwise alignments. Figure S1A shows an identical phylogenetic tree reflecting phylogenetic distances, Figure S1B shows the identical set analysis using maximum likelihood method.
Figure 3.
Comparison of the CcbC, LmbC and LmbC G308V reaction kinetics for various substrates.
The following combinations of proteins and substrates were tested: (A) LmbC vs. L-proline, (B) LmbC vs. EPL, (C) LmbC vs. PPL, (D) LmbC vs. BuPL, (E) LmbC vs. PePL, (F) CcbC vs. L-proline, (G) LmbC G308V vs. L-proline, (H) LmbC G308V vs. EPL and (I) LmbC G308V vs. PPL. All reactions were performed in triplicate. The error bars indicate the standard deviation. The reaction velocity is expressed as the amount of radioactive ATP (μM) produced per minute at protein concentration 0.05 μM. Reaction conditions are described in Experimental Section.
Figure 4.
Homology models of the CcbC and LmbC binding pocket with the substrate.
The models of LmbC (A) and CcbC (B) at frame 805 (time 8.05 ns) of a 20-ns-long, non-restrained MD simulation. Pictures C and D at frame 788 (7.88 ns) and frame 805 (time 8.05 ns) represent another perspective of the CcbC homology model. The letters of the nonribosomal code at upper edge are colored to correspond to the individual amino acid residues of the structures.
Figure 5.
Homology models of the LmbC and LmbC G308V amino acid binding pocket and an RMSD analysis of these models during MD simulations.
Structures of the substrate binding pockets from LmbC (A–C) and LmbC G308V (D–F) homology models with bound PPL during the course of a 20-ns-long, non-restrained MD simulation are shown at 0 ns (left column), 8.05 ns (middle column), and 19.09 ns (right column). The nonribosomal code of each model is displayed at left. The individual letters of the code are colored to correspond to those of the individual amino acids in the structures. A time-based RMSD analysis of the substrate during a 20-ns-long, non-restrained MD simulation of LmbC (blue line) and LmbC G308V (red line). The RMSD was calculated over all substrate C atoms. The positions of the frames 0, 805 and 1909 (corresponding to the time 0 ns, 8.05 ns, and 19.09 ns) are marked with vertical lines.