Table 1.
Primer sequences for H. pylori (Qiagen).
Figure 1.
Relative adhesion (%, related to the untreated control UC) of FITC-labeled H. pylori to AGS cells after pretreatment of the bacteria with different concentrations of Okra FE.
UC: untreated control; Values are mean ± SD; n = 3 independent experiments with 3 replicates each, ** p<0.01.
Figure 2.
Representative images of the adhesion of FITC-labeled H. pylori wild-type strain J99 to immobilized ligands on PVDF membranes.
(I) untreated control and pretreated bacteria with (II) 3′-sialyllactose and (III) Okra FE. (Neo)glycoproteins spotted on PVDF membranes (1 µg per spot) were overlaid with FITC-labeled H. pylori and adherent bacteria were detected by fluorescence imaging. The respective locations of spotted (neo)glycoproteins are indicated below.
Figure 3.
Binding of radiolabeled fractions Okra FE subfractions FE3, 4 and 5 to different H. pylori bacterial strains and E. coli reference strain.
Figure 4.
Influence of Okra FE on H. pylori strain 17875/Leb binding to Leb-HSA.
Data are related to the Leb-HSA binding of the control (only 17875/Leb together with Leb, = 100% Leb binding).
Figure 5.
Influence of Okra FE on H. pylori strain 17875babA1A2 binding to sialyl-Lex-HSA.
Data are related to the control (only 17875babA1A2 with sLex-HAS = 100%).
Figure 6.
Amount of bacteria in the untreated control was calculated as 100% and is indicated as broken line. Results of the samples incubated with MNPs are related to the values of the untreated control. Samples are named according to the type of MNPs added. Results are mean values with ± SD from six independent experiments with *: p<0.05 and **: p<0.01. Significance values refer to the comparison with the fluidMAG-DX® values, as indicated by the brackets.
Figure 7.
Differential gene expression of H. pylori pretreated with Okra FE.
Endogenous control: 23S rRNA. Data are related to untreated control (UC) H. pylori in liquid growth medium (RQ = 1), with n = 2 replicates from three independent experiments (mean ± SD). * p≤0.05