Figure 1.
Divisions and transitions of CSCs/NSCCs as well as kinetics and scheme of the model.
(A). Typical division types of CSCs/NSCCs and transition from NSCC to CSC. Scale bar equals 50 µm. (a–b) Typical in situ division type of a NSCC (white arrow) and transition from a NSCC to a CSC (yellow arrow); (c–d). Typical in situ symmetric divisions of CSCs: self-renewal (one CSC divides into two CSCs) and differentiation (One CSC divides into two NSCCs); NSCC (white arrow), CSC (yellow arrow). (e–f). Typical in situ asymmetric division of CSC (One CSC divide into one CSC and one NSCC); NSCC (white arrow), CSC (yellow arrow). (i). Kinetic equations that correspond to the phenomena in a and b. (ii) Kinetic equations that correspond to the phenomena in c and d. (iii)Kinetic equation that corresponds to the phenomenon in e and f. (B). Scheme of the model based on the experimental results.
Table 1.
Parameters collected from in situ experiments.
Table 2.
Parameters under irradiation surroundings.
Figure 2.
Experiments procedures, results and the simulations of long-term dynamics between CSC and NSCC subpopulations.
(A). Diagram of experiment procedures; (B). Comparison between simulation results and experiment results; (C). Experimental results of long-term equilibrium CSC proportions from initial purified CSCs and NSCCs.
Figure 3.
The long-term dynamics between CSC and NSCC subpopulations via cellular automaton simulation method.
(A). Calculation scheme for cellular automaton method. (B). Typical result of simulation with cellular automaton method (initial condition is that all the cells are NSCCs). Red: CSC; Blue: NSCC; Black: vacant lattice. (C). Comparison between simulation results with cellular automaton method and experimental results.
Figure 4.
Radiation experiments procedures and simulations of long-term dynamics between CSC and NSCC subpopulations.
(A). Diagram of experiment procedures with radiation treatment; (B). Comparison between simulation and experiment results in 0–24 day (radiation is applied when t is 0 day); (C). Amplified image of the results from irradiated 70% CSC group (0–2d) (radiation is applied when t is 0 day).
Figure 5.
Impact on the long-term dynamics between CSC and NSCC subpopulations with sorting error.
(A). Diagram of sorting error in the experiments; (B). Comparison between simulation (KT = 0) and experiment results.