Table 1.
Cinicopathological features of 43 oral squamous cell carcinoma used in this study.
Figure 1.
Keap1 and Nrf2 were overexpressed in human OSCCs.
A, Representative immunohistochemical staining of Keap1 and Nrf2 in human oral cancer tissue (right) compared with normal oral mucosa (left) (Scale bars = 100 umol). B, Quantatitive of histoscore of Keap1 and Nrf2 expression in normal oral mucosa, oral epithelial dysplasia and human oral cancer, Keap1 levels in OSCC was significant higher when compared with oral epithelial dysplasia or normal oral mucosa, and Nrf2 levels in OSCC was significant higher when compared with normal oral mucosa (Mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; One-way ANOVA). C, The expression of Keap1 and Nrf2 were not correlated with lymph node status of human oral cancer (Quantification using Aperio nuclear quantification software, and statistics using Graph Pad Prism 5. Mean ± SEM; *, P<0.05; Mann–Whitney U test). D, Overall survival of the OSCC patients with Keap1 and Nrf2 expression calculated and presented by Kaplan–Meier analysis, and both of Keap1 and Nrf2 expression were not significantly correlated with overall survival (P>0.05).
Figure 2.
Prdx6, PPIA and CD147 were overexpressed in human OSCCs.
A, Representative immunohistochemical staining of Prdx6, PPIA and CD147 in human oral cancer tissue (right) compared with normal oral mucosa (left) (Scale bars = 100 umol). B, Quantatitive of histoscore of Prdx6, PPIA and CD147 expression in normal oral mucosa, oral epithelial dysplasia and human oral cancer, Prdx6, PPIA and CD147 levels in OSCC was significant higher when compared with oral epithelial dysplasia and/or normal oral mucosa (Mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; One-way ANOVA). C, Prdx6, PPIA and CD147 were not significantly associated with lymph node status (Quantification using Aperio nuclear quantification software, and statistics using Graph Pad Prism 5. Mean ± SEM; *, P<0.05; Mann–Whitney U test and One-way ANOVA).
Table 2.
Pearson correlation coefficient test analyses of the array immunostainings of Keap1, Nrf2, PPIA, Prdx6, CD147 in OSCC (n = 43).
Figure 3.
Correlation of Keap1, Nrf2 with PPIA, Prdx6 and CD147 in human OSCC tissue array.
A, The expression of Keap1 had significant correlations with Nrf2 (P = 0.000, R = 0.549), and Prdx6 (P = 0.000, R = 0.735), and CD147 (P = 0.040, R = 0.268) by using the Pearson correlation coefficient test in human OSCC tissue array. B, The expression of Nrf2 was closely correlated with Keap1 (P = 0.000, R = 0.549) and Prdx6 (P = 0.000, R = 0.551) in human OSCC tissue array. C, Hierarchical Clustering of Keap1, Nrf2 with PPIA, Prdx6 and CD147 in human OSCC tissue array. Histoscore based on quantification using Aperio quantification software and statistics with Graph Pad Prism5. Mean ± SEM; 2-tailed Pearson correlation statistics.