Figure 1.
Types and cellular location of proteins identified from bovine adipose tissue.
Mature adipocytes represent the main cellular type in adipose tissue, but other cell types including macrophages, endothelial cells, preadipocytes and stem cells may also be present in adipose tissue.
Figure 2.
Molecular and cellular functions in bovine adipose tissue.
The likelihood of the association between the proteins in the dataset and a biological function is represented as –log(p-value), with larger bars being more significant than shorter bars. The vertical line indicates the cutoff for significance (p-value of 0.05).
Figure 3.
Proteome expression changes due to fat depot and diet.
Changes in expression of 682 proteins are represented by fold change differences (1 to 1.5, 1.5 to 3, 3 to 5, and >5) in adipose tissue collected from different depots in steers fed control of high fat diets: A (Subcutaneous vs. visceral adipose tissue in steers fed Control diet), B (Subcutaneous vs. visceral adipose tissue in steers fed high fat diet), C (Control vs. high fat fed steers using subcutaneous fat tissue), and D (Control vs. high fat fed steers using visceral adipose tissue).
Figure 4.
Integration of bovine miRNAs with proteins identified.
The arrows with dotted lines represent the analysis performed to filter the miRNA and protein datasets. miRNA families highly conserved refer to miRNAs conserved across most vertebrates, miRNA families conserved are conserved across most mammals and miRNA families poorly conserved are not conserved beyond placental mammals.
Figure 5.
Top 10 miRNAs by conservation category predicted to regulate DE proteins between adipose depots involved in lipid metabolism.
The columns indicate the number of proteins each miRNA is predicted to regulate. miRNA families highly conserved refer to miRNAs conserved across most vertebrates, miRNA families conserved are conserved across most mammals and miRNA families poorly conserved are not conserved beyond placental mammals.