Figure 1.
Effects of AVP on HCO3− transport in lMAL and sMAL.
The addition of 10−10 M AVP to the bath decreased HCO3− absorption both in lMALs and sMALs, but the PD increased only in lMALs.
Figure 2.
Time course of HCO3− transport in lMAL and sMAL.
Vehicle was added to the bath after the collection durng the control period. HCO3− transport was stable with time both in lMALs and sMALs, although the PD was decreased only in sMALs.
Table 1.
Effects of AVP, ANP and cGMP on HCO3− transport in sMALs and lMALs.
Figure 3.
Effect of ANP on HCO3− transport in lMALs in the absence of AVP.
ANP at the concentration of 10−8 M did not cause changes in HCO3− absorption in lMALs in the absence of AVP.
Figure 4.
Effects of ANP and cGMP on HCO3− transport in the presence of AVP in lMALs.
ANP at the concentration of 10−10 M and 10−8 M significantly increased HCO3− reabsorption in the presence of 10−10 M AVP in lMALs (A and B, respectively). cGMP at the concentration of 10−4 M mimicked the effect of ANP on HCO3− transport in the presence of 10−10 M AVP in lMALs (C).
Figure 5.
Effect of ANP on HCO3− transport in the presence of AVP in sMALs.
ANP at the concentration of 10−8 M stimulated HCO3− reabsorption in the presence of 10−10 M AVP in sMALs.
Figure 6.
Expression levels of V1aR and V2R mRNA in sMALs and lMALs.
The expression levels of V1aR in sMALs and lMALs were examined using real time PCR. sMALs and lMALs were dissected out of control rats after 30°C in a 0.1% collagenase solution in the presence of VRC. * p<0.05 vs. sMAL, n = 3–5.