Figure 1.
Schematic diagram of fabricating the micro-immunoassay with PS nanofibers substrate.
Figure 2.
Morphologies of electrospun PS fibers examined by SEM.
(A) Superfine fibers with a diameter of 1.0 μm (10000×). (B) and (C) PS fibers’ morphology before and after soaking with aqueous solution (2500×).
Figure 3.
Illustration of the principle of using AZ 5214 to pattern the porous PS nanofiber scaffolds.
(A) Schematic diagram of AZ 5214 penetrating through the porous structure, and bonding with the glass substrate and the surrounding fibers (cross section). (B) and (C) Digital camera pictures of the top view of real products. (D) Micro-chambers filled with different color inks.
Figure 4.
Comparison of fluorescence intensity obtained from different substrates with AFP antigen (100 ng/mL) between blocked and unblocked ones.
Figure 5.
Fluorescence pictures of cancer biomarkers on electrospun PS substrates obtained by an inverted fluorescence microscope (200×).
(A) AFP (DyLight 488, green), (B) CEA (DyLight 405, blue), (C) VEGF (DyLight 649, red); (a-c) light field, (d-f) fluorescence field, (g-i) superposition view of the two fields.
Figure 6.
Relationship between fluorescence intensity and cancer marker concentration on different substrates.