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Figure 1.

Map of Singapore’s remaining primary forest fragments within a secondary forest and urban matrix.

Dark green – primary forest; Light green – secondary forest; red dots – adult K. malaccensis individuals.

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Table 1.

Sample size (N) per locus, size range of alleles, total number of alleles detected, observed (Ho) and expected (He) heterozygosity, genotyping error rate, percent missing data, percent null alleles, and inbreeding co-efficient values (FIS).

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Figure 2.

Allele frequencies for each locus.

Alleles present in very low numbers have the frequency presented above the allele if the bar too small to be easily visualized. All potential allele sizes within the captured range (assuming normal dinucleotide repeat units) are included in the x-axis; blanks indicate that this allele size was not captured. A dot below the allele indicates an unusual size (1 bp difference from nearest allele). ‘a’ indicates a private allele in the adult age class, ‘s’ indicates a private allele in the seedling cohort.

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Table 2.

Comparisons of genetic diversity, reproductive, and spatial genetic structure indices among Singapore K. malaccensis cohorts with reference to Malaysian populations [34], [35].

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Figure 3.

Frequency of different allele frequency classes.

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Figure 4.

Allelic richness accumulation curves for each locus.

Rarefaction curves were produced in the R statistical environment [69].

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Figure 5.

Spatial genetic structure autocorrelograms for adults, saplings, and seedlings, respectively.

‘r’ is the relatedness coefficient of Queller & Goodnight [45] as implemented in GenAlex. Error bars are 95% confidence intervals for the value of r. Upper and lower bounds (dashed lines) represent the 95% confidence intervals for the null hypothesis (no spatial structure).

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