Figure 1.
The flow chart for the isolation and detection of A. acidoterrestris by IMS-PCR.
Figure 2.
The effect of immunocapture time on the separation of A. acidoterrestris in apple juice.
Figure 3.
The effect of quantity of IMPs on the immunocapture of A. acidoterrestris.
Figure 4.
Comparison of the ability of IMS-PCR (a) and PCR (b) to detect A. acidoterrestris in apple juice.
Lane M, DNA markers (100 bp); lines 1-6, 104, 103, 102, 101, 10°, 10-1 CFU/mL; line 7, negative control.
Figure 5.
Specificity of the IMS-PCR assay.
Lane M, DNA markers (100 bp); lines 1-4, A. acidoterrestris (DSM 2498, DSM 3922, DSM 3923, DSM 3924); lines 5-6, A. acidocaldarius (DSM 446, DSM 448); line 7, A. cycloheptanicus (DSM 4006); line 8, A. hesperidum (DSM 12489); line 9, A. herbarius (DSM 13609); line 10, A. acidiphilus (DSM 14558); line 11, A. pomorum (DSM 14955); line 12, A. sendaiensis (DSM 17614); line 13, A. contaminans (DSM 17975); line 14, A. fastidiosus (DSM 17978); line 15, A. acidoterrestris (AAT 13); line 16, A. contaminans (YL-5); line 17, B. subtilis (YL-3); lines 18-19, B. ginsengihumi (LC-8, BS-2); line 20, A. acidoterrestris (C-18); line 21, negative control.