Figure 1.
Gene Expression correlations with Nanostring, ScriptSeq And TruSeq platforms.
Nanostring correlation for (A) undegraded RNA against the same manually degraded sample (RIN=2.0); (B) log2 fold change between two high quality RNAs and the same two samples when manually degraded (RIN 2.0); (C) nine matched fresh-frozen and FFPE pairs. ScriptSeq correlation for (D) undegraded RNA against the same manually degraded sample (RIN=2.0); (E) log2 fold change between two high quality RNAs and the same two samples when manually degraded (RIN 2.0); (F) nine matched fresh-frozen and FFPE pairs.
Figure 2.
Technical Replicates and Cross-Platform Correlations.
Log2 gene expression for FFPE technical replicate with (A) NanoString platform and (B) RiboZeroGold ScriptSeq; (C) Gene expression correlation of nine FFPE samples across nanoString and RiboZeroGold ScriptSeq protocols. Gene expression correlation of nine fresh-frozen (FROZ) RNA samples across (D) nanoString and ScriptSeq, (E) nanoString and TruSeq and (F) RiboZeroGold ScriptSeq and TruSeq.
Figure 3.
RNA-Seq Mapped Reads, Fusion and eSNV statistics By Protocol.
(A) % reads mapped to genome, genes and exon junctions, B) Venn diagram of fusion transcript detection, (C) Number SNV calls, (D) Correlation of FFPE library insert size with sensitivity for single nucleotide variant detection.