Figure 1.
Lulu is required for neural plate hinge point formation.
(A) A four-cell stage embryo viewing from the animal pole. Injections were done in one dorsal animal blastomere (arrowhead). (B) Top views of stage 18 embryos unilaterally injected with indicated morpholinos (MOs, 20 ng) and GFP-Lulu mRNA (25 pg). Asterisks mark the injected side. Squared areas are magnified on the right of each panel. Arrowheads point to the hinge, visible as a pigment line, and brackets indicate weakening or disappearance of the hinge. For quantification of defects, lack of the pigment line was scored as “severe”, and weak or discontinuous pigment line was scored as “mild.” (C) Frequencies of defects shown in (B). (D) Lysates from embryos injected with indicated mRNAs and MOs were subjected to western blot. Ponceau-S staining shows loading.
Figure 2.
Depletion of Lulu disrupts apical constriction in neuroectoderm.
(A, C-D) Transverse sections of the neural plate of stage 17 embryos unilaterally injected with 250 pg of GFP-CAAX mRNA and indicated MOs were stained to visualize GFP and (A) β-catenin, (C) F-actin by phalloidin, (D) phosphorylated myosin light chain (pMLC). Representative images are shown. In (C) and (D), exposure was adjusted to avoid oversaturation of apical staining. Arrows mark the injected side. Dashed lines mark midline. Scale bar: 20 µm. (B) Ratios of apical width to apico-basal cell length of superficial neuroepithelial cells were measured and compared. Up to five cells adjacent to midline were measured per section. Means +/- s. d. are shown. ***: p<0.0001, as compared to the LuluMO group.
Figure 3.
Overexpression of Lulu induces ectopic apical constriction in vivo.
(A) Top views of stage 9 embryos uninjected or injected with 250 pg of GFP-Lulu mRNA. The arrow indicates hyper-pigmented cells. (B) Sections of stage 9 embryos uninjected or injected with GFP-Lulu mRNA. Arrows point to constricting cells. Scale bar: 20 μm. (C) The ratio of apical width to apico-basal cell length of superficial ectodermal cells is shown. Means +/- s. d. are shown. ***: p<0.0001. (D) Sections of stage 9 embryos injected with GFP-Lulu mRNA were stained with anti-GFP antibody and phalloidin. Superficial ectodermal cells are shown. Note the increase of apical and junctional staining of phalloidin in cells expressing GFP-Lulu (arrowheads). Scale bar: 20 μm.
Figure 4.
The FERM and FA domains of Lulu are necessary for the induction of apical constriction.
(A) Schematic illustration of various deletion mutants of Lulu. Amino acid numbers are indicated. (B) Embryos were injected with 250 pg of mRNAs encoding indicated Lulu mutants. Percentage of the embryos showing ectopic hyper-pigmentation at stage 9 was shown. Phenotypes were scored based on the degree of pigmentation increase, and representative images are shown on the right side. (C) Top views of stage 9 embryos injected with indicated mRNAs. (D) Sections of stage 9 embryos injected with indicated mRNAs were stained with anti-GFP and anti-β-catenin antibodies. Superficial ectodermal cells are shown. Note the change of cell shape shown by the staining of β-catenin. Scale bar: 20 μm.
Figure 5.
The carboxy-terminal fragment of Lulu functions as a dominant-negative mutant.
(A) Top views of stage 9 embryos injected with indicated mRNAs. 100 pg of GFP-Lulu mRNA and 5 ng of GFP-Lulu-C or Flag-Lulu-C mRNAs were injected. Arrows point to ectopic hyper-pigmentation. (B) Percentage of the embryos showing ectopic hyper-pigmentation. Phenotypes were scored as described in Figure 4 legend. (C) Lysates from embryos injected with indicated mRNAs were subjected to Western Blot. α-tubulin serves as the loading control. (D) Sections of stage 9 embryos injected with indicated mRNAs were stained with anti-GFP and anti-flag antibodies. Superficial ectodermal cells are shown. Note the change of cell shape shown by the staining of GFP. Arrows indicate constricting cells. (E) Top views of stage 17 embryos uninjected or unilaterally injected with 5 ng of Lulu-C mRNA. Asterisks mark the injected side. Arrowheads point to the hinge of neural plate, and brackets indicate disappearance of the hinge. (F) Transverse sections of the neural plate of stage 17 embryos, unilaterally injected with GFP-Lulu-C mRNAs, were stained to visualize GFP, β-catenin, and F-actin. Dashed lines mark midline. Scale bar: 20 μm.
Figure 6.
Lulu activity is required for Shroom3 to induce apical constriction.
(A) Top views of stage 9 embryos injected with indicated mRNAs and MOs. 50 pg of Myc-Shroom3 (Myc-Shrm3) or ShroomS (ShrmS) mRNAs and 20 ng of MOs were injected. Arrows point to ectopic hyper-pigmentation. (B) Percentage of the embryos showing the constriction phenotype. Phenotypes were scored as described in Figure 4 legend. (C) Lysates from embryos injected with indicated mRNAs and MOs were subjected to western blotting. α-tubulin serves as the loading control. (D) Sections of stage 9 embryos injected with indicated mRNAs and MOs were stained with anti-Myc antibodies. Superficial ectodermal cells are shown. Arrows point to constricting cells. Scale bar: 20 μm.
Figure 7.
Lulu synergizes with Shroom3 to induce apical constriction.
(A) Top views of stage 9 embryos injected with indicated mRNAs. 25 pg of GFP-Lulu mRNA and 10 pg of Myc-Shroom3 (Shrm3) mRNA were injected. Arrows point to ectopic hyper-pigmentation. (B, C) Sections of stage 9 embryos injected with indicated mRNAs were stained with anti-Myc (B) and anti-GFP antibodies (C). Superficial ectodermal cells are shown. Arrows point to constricting cells. Scale bar: 20 μm.