Table 1.
Cd-responsive miRNAs identified by microarray analysis.
Figure 1.
Expression patterns of 16 Cd-responsive miRNAs confirmed by qRT-PCR.
The y-axis represents the expression levels of Cd/CK. The dark grey shading bar represents the relative expression level of miRNAs in HX3 measured by microarray in responsive to Cd stress. The dark grey diagonal stripe shading bar represents the relative expression level of miRNAs in ZH24 measured by microarray in responsive to Cd stress. The french grey shading bar represents the relative expression level of miRNAs in HX3 measured by qRT-PCR in responsive to Cd stress. The french grey diagonal stripe shading bar represents the relative expression level of miRNAs in ZH24 measured by qRT-PCR in responsive to Cd stress. The results of qRT-PCR are mean ± SD of duplicates of three biological replicates. Significance of the changes between HX3 and ZH24 at the levels of Cd/CK was checked with Student's t-test at the level of P≤0.01 (shown as “**”).
Figure 2.
T-plots of the targets cleaved by the miR398 family.
The T-plots show the distribution of the degradome tags along the full-length of the target mRNA sequence (bottom). The red line represents the sliced target trancripts and is shown in arrow. The alignment shows the miRNA with a portion of its target sequence (top). The two dots indicate matched RNA base pairs; one dot indicates a GU mismatch. The arrow shows the cleavage site. (A) Examples of copper/zinc superoxide dismutase as target for gma-miR398a. (B) Examples of prolyl oligopeptide family protein as target for gma-miR398c. (C) Examples of zinc finger transcription factor as target for gma-miR398c. (D) Examples of blue-copper-binding protein as target for gma-miR398c.
Figure 3.
GO analyses of the targets of the 14 Cd-stress-responsive miRNAs in soybean.
The dark grey shading bars indicate the enrichment of the GO terms in the miRNA targets in GO. The french grey shading bars indicate the percentage of the total annotated soybean genes that were mapped to the GO terms.
Table 2.
Target genes of 14 Cd-responsive miRNAs and their functional annotation.
Figure 4.
Expression profiles of target genes of Cd-responsive miRNAs between HX3-CK and HX3-Cd.
The expression levels of mRNAs were normalized to the level of F-box. The results are mean ± SD of the duplicates of three biological replicates. Significance of the changes between HX3-CK and HX3-Cd was checked with Student's t-test at the level of 0.01<P≤0.05 (shown as “*”) and P≤0.01 (shown as “**”).
Figure 5.
Expression profiles of target genes of Cd-responsive miRNAs between ZH24-CK and ZH24-Cd.
The expression levels of mRNAs were normalized to the level of F-box. The results are mean ± SD of the duplicates of three biological replicates. Significance of the changes between ZH24-CK and ZH24-Cd was checked with Student's t-test at the level of 0.01<P≤0.05 (shown as “*”) and P≤0.01 (shown as “**”).