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Table 1.

Presence of Bluetongue virus RNA and BTV antibodies in blood samples of ewes on different days post inoculation.

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Table 1 Expand

Table 2.

Presence of Bluetongue infectious virus or RNA in foetal tissues: BTV 8 infected group+commingled control.

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Table 2 Expand

Table 3.

Presence of Bluetongue infectious virus or RNA in foetal tissues: BTV-1 infected group+commingled control.

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Table 3 Expand

Figure 1.

Normal foetal brain with normally developed cerebral gyri.

Foetus 78654.2 (BTV-8 group, VI/PCR positive).

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Figure 1 Expand

Figure 2.

Localized cystic defect in the cerebral hemisphere (right frontal lobe).

Foetus 78630.2 (BTV-1 group, VI/PCR positive).

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Figure 2 Expand

Figure 3.

Hydranencephaly.

The cerebrum is transformed to fluid filled sacs with only focal presence of cerebral tissue at the periphery. Foetus 78631.3 (BTV-1 group, VI/PCR positive).

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Figure 3 Expand

Figure 4.

Incidence ratio of microscopic findings.

Incidence of lesion per number of foetuses for the BTV-8 positive foetuses (n = 14), BTV-1 positive foetuses (n = 14) and BTV negative foetuses (n = 23).

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Figure 4 Expand

Figure 5.

Meningitis.

Increase of meninges thickness (A) due to mononuclear infiltrate, necrosis (arrow) and presence of giant cells(circle). Cerebrum (10×10) of foetus 78649.1 (BTV-1 group, VI/PCR positive).

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Figure 5 Expand

Figure 6.

Mononuclear inflammatory infiltrates (circles).

Cerebrum (4×10) of foetus 78649.1 (BTV-1 group, VI/PCR positive).

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Figure 6 Expand

Figure 7.

Necrosis and liquefaction of cerebral tissue.

Cerebrum (10×10) of foetus 78655.1 (BTV-1 group, VI/PCR positive).

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Figure 7 Expand

Figure 8.

Necrosis, presence of multiple multinucleated giant cells (arrows), the lower one with vacuoles.

Cerebrum (40×10) of foetus 78655.1 (BTV-1 group, VI/PCR positive).

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Figure 8 Expand

Figure 9.

Normally developed cerebral tissue.

Cerebrum (10×10) of foetus 78626.3(control of BTV-1 group, VI/PCR negative).

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Figure 9 Expand

Figure 10.

Ballooning degeneration of epithelium (arrow) and congestion of blood vessels.

Plexus choroideus (20×10) of foetus 78631.3 (BTV-1 group, VI/PCR positive).

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Figure 10 Expand