Figure 1.
Size distribution of assembled C. pentagona (C. pen) and C. suaveolens (C. sua) ESTs.
The X-axis depicts the sizes of ESTs divided in 200 nt increments. ESTs smaller than 200 nt were excluded, and those large then 4,000 nt were combined. The Y-axis represents the number of ESTs falling into each size categories.
Table 1.
C. pentagona and C. suaveolens transcriptome statistics.
Table 2.
Comparison of putative dodder proteins with proteins of other plants.
Figure 2.
The lengths of C. pentagona (C. pen) and C. suaveolens (C. sua) ESTs as estimated by the calculating ortholog hit ratio (OHR; see text for detailed description).
ESTs with OHR approaching 1 are considered likely to be of full lengths.
Figure 3.
Proportion of dodder ESTs sharing the highest similarities with mRNAs of each of the twelve non-parasitic plant species.
Figure 4.
Gene Ontology categories of assembled dodder ESTs based on biological functions, with the transcriptome of sweet potato as reference.
The various GO categories are listed along the X-axis, whereas the Y-axes (both sides) represent two different ways to showcase the number of ESTs in each GO category. On the left side, the numbers were converted to percentages of the total annotated ESTs, whereas on the right side actual numbers were plotted. Note that the scales on both Y-axes are logarithmic.
Table 3.
Cryptic virus signatures in dodder sequence libraries.
Table 4.
Candidate ESTs with possible roles in the parasitic life style.
Figure 5.
Confirmation of four pairs of dodder ESTs with RT-PCR.
Four pairs of C. pentagona (C. pen) and C. suaveolens (C. sua) ESTs were chosen for assessment, generating fragments of 342, 392, 296, and 405 bps, respectively. Sequences of the primers used are provided in Section II of Text S1.