Figure 1.
(a) Cartoon representation of the human G3BP1 NTF2-like domain. Chain A: skyblue, chain B: slate (b) Dimer interface, chain A. (c) Dimer interface, chain B (color coding for interface residues; grey: hydrophobic, red: acidic, blue: basic, white: polar).
Table 1.
Data reduction and refinement statistics.
Figure 2.
Crystal packing and implications.
(a) Cartoon representation of binding of N-terminal residues from a symmetry related molecule (grey) to chain A (skyblue). (b) Close-up of the binding of N-terminal residues. (c) Manual docking of four residues, residues 378–381, from the minimum binding motif of Caprin-1.
Figure 3.
Isothermal calorimetric titrations for binding of Nup peptides to the G3BP1 NTF2-like domain.
(a) Titration of the FxFG peptide to G3BP1 1–139 (upper panel: raw data, lower panel integrated heat of binding). (b) Titration of FG peptide into G3BP1 1–139 (raw data). (c) Titration of GLFG peptide into G3BP1 1–139 (raw data). (d) Titration of FxFG peptide to the G3BP1 1–139 Phe15Ala mutant (upper panel: raw data, lower panel: integrated heat of binding).
Figure 4.
(a) Cartoon representation of the peptide complex. The FxFG peptide is shown as pink sticks. The positions of an FxFG peptide in yeast nuclear transport factor 2 (yellow sticks, PDB ID 1gyb) and an FG peptide in the TAP-p15 complex (gray sticks, PDB ID 1jn5) are indicated. (b) Close up of the peptide binding site. 2Fo-Fc electron density map contoured at sigma 1 is shown for the FxFG peptide and hydrogen binding and salt bridge distances are given in Ångstrom. (c) Schematic LIGPLOT overview of interactions between G3BP1 (blue) and the FxFG peptide (pink). Hydrogen bonds are indicated by black dashed lines, hydrophobic contacts are represented by arcs with spokes radiating towards the peptide and pi-stacking is indicated by red dashed lines. (d) Alignment of the FxFG peptides from chain A (pink) and chain B (light pink).
Figure 5.
Surface representation of nuclear transport factor 2 (left hand side, PDB ID 1a2k) and the G3BP1 NTF2-like domain (right hand side, PDB ID 4fcj) colored by Eisenberg`s hydrophobicity scale [50]. Phe72 of RanGDP is shown as sticks (green). The corresponding proposed binding sites for Ran at G3BP are indicated by solid arrows. The binding site for the FxFG peptide is indicated by a dashed arrow.