Figure 1.
Assay of drought resistance of the two cotton cultivars.
(A) Seedlings of the cultivar J-13 in pot after withholding water for 15 days. (B) Seedlings of the cultivar Lu-6 in pot after withholding water for 15 days. (C) The percentage of wilting seedlings after withholding water for 13 and 15 days. J-13, cotton cultivar Jingmian13; Lu-6, cotton cultivar Lumian6.
Figure 2.
Effects of drought stress on lipid perxidation, electronlyte leakage, POD and CAT activity in the leaves of cotton cultivars J-13 and Lu-6.
All the values are mean ±S.D. Independent t-tests demonstrated that there was significant (* p<0.05) or very significant (** p<0.01) difference between the drought-treated samples and controls, and there was significant (∮p<0.05) or very significant (∮∮ p<0.01) difference between J-13 and Lu-6 at the same drought stress time point. Error bars were calculated from three independent experiments.
Figure 3.
Histogram representation of clusters of orthologous groups (COG) classification.
Figure 4.
The percentage of each GO term in the GO classification.
(A) The Biological Process percentage. (B) The Cellular Component percentage. (C) The Molecular Function percentage.
Figure 5.
Expression profiles of the isolated genes in leaves of cotton J-13 seedlings under drought stress.
Relative expression levels were determined by real-time quantitative RT-PCR for data verification. Error bars represent the SD for three independent experiments, and three technical replicates were analyzed. Jck, J1d, J3d, J4d, J5d, J6d and J7d on the x-axis refer to cotton J-13 seedlings after withholding water for 0 (control), 1, 3, 4, 5, 6, and 7 days. The y-axis represents the relative expression level.
Figure 6.
GO functional classification of cotton J-13.
GO functional classification analysis between control and 2, 4, 6 and 8 day-drought samples. Histograms represent the functional distribution, which is expressed as a percentage of the amount of genes.
Figure 7.
GO functional classification of two different samples.
GO functional classification analysis of differentially expressed genes between J-13 and Lu-6 after 6 days of drought treatment. Histograms represent the functional distribution, which is expressed as a percentage of the amount of genes.