Figure 1.
Schematic representation of the sampling compartments in RAS.
Water circuit of the recirculating aquaculture system studied (not to scale).
Figure 2.
Ordination diagrams (PCO) of the bacterial community based on DGGE profile.
A) in all sampling compartments (water supply (Sup), turbot production tank (TurPro), turbot sedimentation tank (TurSed), turbot biofilter tank (TurBio), turbot ozone tank (TurOzo), sole production tank (SolPro), sole sedimentation tank (SolSed), sole biofilter tank (SolBio), sole ozone tank (SolOzo)); B) only in turbot and sole sampling compartments (without water supply).
Figure 3.
Rarefied OTU richness in all sampling compartments (water supply (Sup), turbot production tank (TurPro), turbot sedimentation tank (TurSed), turbot biofilter tank (TurBio), turbot ozone tank (TurOzo), sole production tank (SolPro), sole sedimentation tank (SolSed), sole biofilter tank (SolBio), sole ozone tank (SolOzo)).
Figure 4.
Ordination diagrams (PCO) of the bacterial community based on barcoded pyrosequencing data.
(A) in all sampling compartments; (B) the most abundant OTU’s associated to all sampling points; (C) only in turbot and the sole sampling compartments (without water supply); (D) the most abundant OTUs associated to turbot and the sole sampling compartments.
Figure 5.
Relative abundance of the most dominant bacterial groups (2 phyla, 8 classes and 10 orders) in each sampling compartments (water supply (Sup), turbot production tank (TurPro), turbot sedimentation tank (TurSed), turbot biofilter tank (TurBio), turbot ozone tank (TurOzo), sole production tank (SolPro), sole sedimentation tank (SolSed), sole biofilter tank (SolBio), sole ozone tank (SolOzo)). Groups are present from the most abundant to the least abundant.
Figure 6.
Neighbour-joining phylogenetic tree (16S rRNA gene sequences) of the most dominant OTUs in turbot and sole RAS and their closest relatives (accession number-species).
Bootstrap values generated from 500 replicates. Values lower than 50% were omitted.
Figure 7.
Neighbour-joining phylogenetic tree (16S rRNA gene sequences) of OTUs related potential fish pathogens and their closest relatives (with accession number).
Black circles indicate the most frequent pathogens described in literature. Bootstrap values generated from 500 replicates. Values lower than 50% were omitted.